caa a22 4500 606190805 CHVBK 20210128100857.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10577-015-9466-8 doi (NATIONALLICENCE)springer-10.1007/s10577-015-9466-8 The differential loading of two barley CENH3 variants into distinct centromeric substructures is cell type- and development-specific [Elektronische Daten] [Takayoshi Ishii, Raheleh Karimi-Ashtiyani, Ali Banaei-Moghaddam, Veit Schubert, Jörg Fuchs, Andreas Houben] The organization of centromeric chromatin of diploid barley (Hordeum vulgare) encoding two (α and β) CENH3 variants was analysed by super-resolution microscopy. Antibody staining revealed that both CENH3 variants are organized in distinct but intermingled subdomains in interphase, mitotic and meiotic centromeres. Artificially extended chromatin fibres illustrate that these subdomains are formed by polynucleosome clusters. Thus, a CENH3 variant-specific loading followed by the arrangement into specific intermingling subdomains forming the centromere region appears. The CENH3 composition and transcription vary among different tissues. In young embryos, most interphase centromeres are composed of both CENH3 variants, while in meristematic root cells, a high number of nuclei contain βCENH3 mainly dispersed within the nucleoplasm. A similar distribution and no preferential arrangement of the two CENH3 variants in relationship to the spindle poles suggest that both homologs meet the same function in metaphase cells. © European Union, 2015 CENH3 variants nationallicence centromere substructure nationallicence Hordeum vulgare nationallicence nucleosomes nationallicence CENH3 loading nationallicence CENH3 : Centromeric histone H3 variant nationallicence CENP-C : Centromere protein C nationallicence SIM : Structured illumination microscopy nationallicence Ishii Takayoshi Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut Karimi-Ashtiyani Raheleh Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut Banaei-Moghaddam Ali Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut Schubert Veit Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut Fuchs Jörg Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut Houben Andreas Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut Chromosome Research Springer Netherlands 23/2(2015-06-01), 277-284 0967-3849 23:2<277 2015 23 10577 https://doi.org/10.1007/s10577-015-9466-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10577-015-9466-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishii Takayoshi Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut NATIONALLICENCE 700 1- Karimi-Ashtiyani Raheleh Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut NATIONALLICENCE 700 1- Banaei-Moghaddam Ali Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut NATIONALLICENCE 700 1- Schubert Veit Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut NATIONALLICENCE 700 1- Fuchs Jörg Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut NATIONALLICENCE 700 1- Houben Andreas Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Corrensstr. 3, 06466, Stadt Seeland, Germany aut NATIONALLICENCE 773 0- Chromosome Research Springer Netherlands 23/2(2015-06-01), 277-284 0967-3849 23:2<277 2015 23 10577