caa a22 4500 606193561 CHVBK 20210128100910.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10682-015-9779-2 doi (NATIONALLICENCE)springer-10.1007/s10682-015-9779-2 Increased divergence in floral morphology strongly reduces gene flow in sympatric sexually deceptive orchids with the same pollinator [Elektronische Daten] [Julia Gögler, Johannes Stökl, Pierluigi Cortis, Heinrich Beyrle, Maria Barone Lumaga, Salvatore Cozzolino, Manfred Ayasse] In sexually deceptive orchids, pollinator specificity is the main factor responsible for species isolation. The two sexually deceptive orchids Ophrys chestermanii and Ophrys normanii occur sympatrically on Sardinia and attract the same pollinator, males of Bombus vestalis, by producing the same odour bouquets. Since gene flow between these two species has been shown to be almost absent, their genetic distinctness seems to be preserved by other forms of reproductive barrier than pollinator specificity. The aim of this study is to investigate the nature and strength of these isolation barriers which apparently halt gene flow between these two orchids that share the same pollinator. Morphological measurements, combined with observations of cross-pseudocopulations, revealed that, in sympatry, the longer caudicles of O.normanii and the smaller and differently shaped stigmatic cavity in O.chestermanii are the main factors preventing gene flow between these two sympatric species. In contrast, other pre- or post-mating barriers seem to be weak or absent, as the two species have overlapping flowering time and we were able to rear hybrid offspring from interspecific manual crosses. At the same time, genetic analysis of orchid plantlets collected at sympatric sites detected the presence of only two putative hybrid individuals. Thus, the probability of natural cross-pollination between O.normanii and O.chestermanii seems to be strongly limited by divergence in their floral morphology. For some traits, this divergence was found increased between sympatric populations. Springer International Publishing Switzerland, 2015 Ecological character displacement nationallicence Ophrys chestermanii nationallicence Ophrys normanii nationallicence Phenology nationallicence Pollinia placement nationallicence Mechanical barriers nationallicence Reinforcement nationallicence Reproductive isolation nationallicence Gögler Julia Institute for Experimental Ecology, University of Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany aut Stökl Johannes Institute of Zoology, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany aut Cortis Pierluigi Department of Life and Environmental Sciences, University of Cagliari, Viale S. Ignazio 13, 09123, Cagliari, Italy aut Beyrle Heinrich Myorchids Ltd., Lindenmahdstr. 19, 86382, Stadtbergen, Germany aut Barone Lumaga Maria Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy aut Cozzolino Salvatore Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy aut Ayasse Manfred Institute for Experimental Ecology, University of Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany aut Evolutionary Ecology Springer International Publishing 29/5(2015-09-01), 703-717 0269-7653 29:5<703 2015 29 10682 https://doi.org/10.1007/s10682-015-9779-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10682-015-9779-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gögler Julia Institute for Experimental Ecology, University of Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany aut NATIONALLICENCE 700 1- Stökl Johannes Institute of Zoology, University of Regensburg, Universitätsstraße 31, 93053, Regensburg, Germany aut NATIONALLICENCE 700 1- Cortis Pierluigi Department of Life and Environmental Sciences, University of Cagliari, Viale S. Ignazio 13, 09123, Cagliari, Italy aut NATIONALLICENCE 700 1- Beyrle Heinrich Myorchids Ltd., Lindenmahdstr. 19, 86382, Stadtbergen, Germany aut NATIONALLICENCE 700 1- Barone Lumaga Maria Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy aut NATIONALLICENCE 700 1- Cozzolino Salvatore Department of Biology, University of Naples Federico II, Via Cinthia, 80126, Naples, Italy aut NATIONALLICENCE 700 1- Ayasse Manfred Institute for Experimental Ecology, University of Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany aut NATIONALLICENCE 773 0- Evolutionary Ecology Springer International Publishing 29/5(2015-09-01), 703-717 0269-7653 29:5<703 2015 29 10682