caa a22 4500 606193588 CHVBK 20210128100910.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10682-015-9785-4 doi (NATIONALLICENCE)springer-10.1007/s10682-015-9785-4 Mosaic genetic differentiation along environmental and geographic gradients indicate divergent selection in a white pine species complex [Elektronische Daten] [Alejandra Moreno-Letelier, Timothy Barraclough] We explored the role of isolation by environment in a white pine species complex: Pinus flexilis, Pinus strobiformis and Pinus ayacahuite distributed from Canada to Central America. We predict that species differentiation would match genetic structure of candidate genes associated with significant differences in climatic niche in the species complex. To test this prediction, we sequenced five candidate genes for drought tolerance and three housekeeping genes, in individuals from across the entire range of each species. We performed neutrality tests, estimated genetic differentiation and performed partial mantel correlations, to test for isolation by environment in the species complex. Our results show that different loci vary in degrees of genetic differentiation within species and contrast in patterns of differentiation among species. This is considered to be a mosaic pattern of genetic differentiation. There was also significant isolation by environment in candidate genes. P. flexilis was genetically differentiated for candidate genes and P. ayacahuite for housekeeping genes. There was also an overall pattern of shared ancestral polymorphism followed by independent evolution. Nonetheless, all loci together recovered groups that correspond to the recognized taxonomy. In conclusion, the pattern of isolation by environment in candidate genes support the idea of ecologically driven differentiation of this species complex, especially in the case of P. flexilis. The observed difference in housekeeping genes between P. strobiformis and P. ayacahuite can be due to limited gene flow. The mosaic pattern of differentiation suggests that speciation is recent and ecological differences could be a factor in the diversification of pines in North America. Springer International Publishing Switzerland, 2015 Speciation nationallicence Divergent selection nationallicence Isolation by environment nationallicence Pinus nationallicence Genetic structure nationallicence Moreno-Letelier Alejandra Department of Life Sciences, Imperial College London, Silwood Park Campus, Buckhurst Road, SL5 7PY, Ascot, Berkshire, UK aut Barraclough Timothy Department of Life Sciences, Imperial College London, Silwood Park Campus, Buckhurst Road, SL5 7PY, Ascot, Berkshire, UK aut Evolutionary Ecology Springer International Publishing 29/5(2015-09-01), 733-748 0269-7653 29:5<733 2015 29 10682 https://doi.org/10.1007/s10682-015-9785-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10682-015-9785-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moreno-Letelier Alejandra Department of Life Sciences, Imperial College London, Silwood Park Campus, Buckhurst Road, SL5 7PY, Ascot, Berkshire, UK aut NATIONALLICENCE 700 1- Barraclough Timothy Department of Life Sciences, Imperial College London, Silwood Park Campus, Buckhurst Road, SL5 7PY, Ascot, Berkshire, UK aut NATIONALLICENCE 773 0- Evolutionary Ecology Springer International Publishing 29/5(2015-09-01), 733-748 0269-7653 29:5<733 2015 29 10682