caa a22 4500 606193677 CHVBK 20210128100910.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10682-015-9753-z doi (NATIONALLICENCE)springer-10.1007/s10682-015-9753-z Strong postzygotic isolation prevents introgression between two hybridizing Neotropical orchids, Epidendrum denticulatum and E. fulgens [Elektronische Daten] [Fábio Pinheiro, Poliana Cardoso-Gustavson, Rogério Suzuki, Monique Abrão, Leonardo Guimarães, David Draper, Ana Moraes] Studies on hybrid zones are essential to understand the origin and evolution of reproductive barriers in plants. To achieve this goal, multidisciplinary approaches are often required to investigate the role of multiple reproductive isolation (RI) mechanisms. For Epidendrum denticulatum and E. fulgens, two Neotropical food-deceptive orchid species, we used molecular, cytogenetic and morphological analyses, experimental crosses and environmental envelope models to assess the strength of the RI and the mechanisms that prevent species collapse when hybridization occurs. Based on genetic assignment tests, hybrids between E. denticulatum and E. fulgens were detected. However, the low frequency of hybrid specimens found, coupled with the high morphological differentiation between parental species, suggested that strong barriers exist to interspecific gene exchange. Indeed, hybrid plants were largely sterile, as determined by meiotic data and crossing experiments. In the hybrid zone studied here, strong postzygotic barriers maintain species integrity, and these RI mechanisms may be also important during early stages of speciation. Springer International Publishing Switzerland, 2015 Hybridization meiotic behavior nationallicence Orchidaceae nationallicence Environmental envelope models nationallicence Reproductive isolation nationallicence Speciation nationallicence Pinheiro Fábio Departamento de Botânica, Instituto de Biociências, Universidade Estadual Paulista (UNESP), 13506-900, Rio Claro, SP, Brazil aut Cardoso-Gustavson Poliana Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut Suzuki Rogério Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut Abrão Monique Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut Guimarães Leonardo Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut Draper David Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, CP 11 01 608, Loja, Ecuador aut Moraes Ana Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista (UNESP), 18618-970, Botucatu, SP, Brazil aut Evolutionary Ecology Springer International Publishing 29/2(2015-03-01), 229-248 0269-7653 29:2<229 2015 29 10682 https://doi.org/10.1007/s10682-015-9753-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10682-015-9753-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pinheiro Fábio Departamento de Botânica, Instituto de Biociências, Universidade Estadual Paulista (UNESP), 13506-900, Rio Claro, SP, Brazil aut NATIONALLICENCE 700 1- Cardoso-Gustavson Poliana Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Suzuki Rogério Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Abrão Monique Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Guimarães Leonardo Instituto de Botânica, Núcleo de Pesquisa do Orquidário do Estado, 04301-902, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Draper David Departamento de Ciencias Naturales, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, CP 11 01 608, Loja, Ecuador aut NATIONALLICENCE 700 1- Moraes Ana Departamento de Genética, Instituto de Biociências, Universidade Estadual Paulista (UNESP), 18618-970, Botucatu, SP, Brazil aut NATIONALLICENCE 773 0- Evolutionary Ecology Springer International Publishing 29/2(2015-03-01), 229-248 0269-7653 29:2<229 2015 29 10682