caa a22 4500 606194754 CHVBK 20210128100915.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10934-014-9864-x doi (NATIONALLICENCE)springer-10.1007/s10934-014-9864-x Pore size analysis from low field NMR spin-spin relaxation measurements of porous microspheres [Elektronische Daten] [Xiunan Li, Yaqiong Li, Chao Chen, Dawei Zhao, Xiaojun Wang, Lan Zhao, Hong Shi, Guanghui Ma, Zhiguo Su] The porous structure characteristics of twenty-one porous microspheres with agarose framework were investigated based on the low-field nuclear magnetic resonance (LF NMR) relaxation time (T 2) distribution measurements. The feasibility of the technique was confirmed by direct relationship between T 2 and the mean pore size of the polymer networks (Rh) of agarose hydrogel which was established using the "Fiber-Cell” model. The expected pore radius distribution curve was obtained and the reliability was validated by comparing them with the results obtained by inverse size-exclusion chromatography. Thereafter, the technique was applied to characterize the pore size distribution (PSD) of soft microspheres and the pore size transformation of microspheres with or without grafted polymers in the process of protein adsorption. All of these results strongly support LF NMR as a promising, rapid and nondestructive technique for the determination of PSDs in many kinds of soft porous microspheres. Springer Science+Business Media New York, 2014 LF NMR nationallicence Transverse relaxation time T 2 nationallicence Porous microspheres nationallicence Pore size distribution (PSD) nationallicence Li Xiunan National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Li Yaqiong West China School of Pharmacy, Sichuan University, 610041, Chengdu, China aut Chen Chao National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Zhao Dawei National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Wang Xiaojun College of Food and Bioengineering, Shandong Polytechnic University, 250353, Jinan, China aut Zhao Lan National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Shi Hong National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Ma Guanghui National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Su Zhiguo National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut Journal of Porous Materials Springer US; http://www.springer-ny.com 22/1(2015-02-01), 11-20 1380-2224 22:1<11 2015 22 10934 https://doi.org/10.1007/s10934-014-9864-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10934-014-9864-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiunan National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Li Yaqiong West China School of Pharmacy, Sichuan University, 610041, Chengdu, China aut NATIONALLICENCE 700 1- Chen Chao National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Zhao Dawei National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Wang Xiaojun College of Food and Bioengineering, Shandong Polytechnic University, 250353, Jinan, China aut NATIONALLICENCE 700 1- Zhao Lan National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Shi Hong National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Ma Guanghui National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 700 1- Su Zhiguo National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 100190, Beijing, China aut NATIONALLICENCE 773 0- Journal of Porous Materials Springer US; http://www.springer-ny.com 22/1(2015-02-01), 11-20 1380-2224 22:1<11 2015 22 10934