caa a22 4500 606195823 CHVBK 20210128100921.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10934-015-9998-5 doi (NATIONALLICENCE)springer-10.1007/s10934-015-9998-5 Bi-layered porous constructs of PCL-coated 45S5 bioactive glass and electrospun collagen-PCL fibers [Elektronische Daten] [Preethi Balasubramanian, Judith Roether, Dirk Schubert, Justus Beier, Aldo Boccaccini] A simple yet promising approach to construct bi-layered scaffolds using bioactive ceramics and biodegradable polymers is presented. This method involves two versatile fabrication techniques used in the field of TE: foam replication process and electrospinning. By the foam replication method, three-dimensional 45S5 bioactive glass (BG)-based scaffolds with high porosity, in the range of 95.8±0.9%, were produced. To improve the mechanical properties of the BG scaffolds, dip-coating using polycaprolactone (PCL) was performed, which led to a significant increase in the compressive strength of the scaffolds. In order to develop a bi-layered construct, bead-less submicrometric fibers of collagen-PCL were electrospun over the PCL-coated BG scaffolds. Surface morphology, surface properties and mechanical strength of the bi-layered construct were evaluated using scanning electron microscopy analysis, contact angle measurements and compressive strength testing, respectively. In vitro degradation of the collagen-PCL fibers in phosphate buffered saline and in vitro bioactivity of the bi-layered constructs in simulated body fluid were investigated. Formation of hydroxyapatite on the PCL-coated BG and along the morphology of the collagen-PCL fibers was ascertained using different characterization techniques. The bi-layered construct is intended for interface tissue engineering applications where the PCL-coated BG scaffold, which is highly bioactive, can serve as a support for the bone side and the composite collagen-PCL submicrometric fibers are intended for the cartilage side. Springer Science+Business Media New York, 2015 Osteochondral region nationallicence Electrospinning nationallicence In vitro degradation nationallicence Bioactivity nationallicence Scaffold nationallicence Balasubramanian Preethi Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany aut Roether Judith Department of Materials Science and Engineering, Institute of Polymer Materials, University of Erlangen-Nuremberg, Martenstr. 7, 91058, Erlangen, Germany aut Schubert Dirk Department of Materials Science and Engineering, Institute of Polymer Materials, University of Erlangen-Nuremberg, Martenstr. 7, 91058, Erlangen, Germany aut Beier Justus Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital Erlangen, University of Erlangen-Nuremberg, Erlangen, Germany aut Boccaccini Aldo Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany aut Journal of Porous Materials Springer US; http://www.springer-ny.com 22/5(2015-10-01), 1215-1226 1380-2224 22:5<1215 2015 22 10934 https://doi.org/10.1007/s10934-015-9998-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10934-015-9998-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Balasubramanian Preethi Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Roether Judith Department of Materials Science and Engineering, Institute of Polymer Materials, University of Erlangen-Nuremberg, Martenstr. 7, 91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Schubert Dirk Department of Materials Science and Engineering, Institute of Polymer Materials, University of Erlangen-Nuremberg, Martenstr. 7, 91058, Erlangen, Germany aut NATIONALLICENCE 700 1- Beier Justus Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital Erlangen, University of Erlangen-Nuremberg, Erlangen, Germany aut NATIONALLICENCE 700 1- Boccaccini Aldo Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany aut NATIONALLICENCE 773 0- Journal of Porous Materials Springer US; http://www.springer-ny.com 22/5(2015-10-01), 1215-1226 1380-2224 22:5<1215 2015 22 10934