caa a22 4500 60619617X CHVBK 20210128100923.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10934-014-9905-5 doi (NATIONALLICENCE)springer-10.1007/s10934-014-9905-5 Impact of structural features of SBA-15 host particles on activity of immobilized glucose oxidase enzyme and sensitivity of a glucose sensor [Elektronische Daten] [Anees Khan, Santosh Noronha, Rajdip Bandyopadhyaya] Glucose oxidase (GOD) enzyme immobilized mesoporous silica particles (SBA-15) have been developed as an amperometric glucose sensor. SBA-15 particles with fibre, rod and cuboid morphologies of decreasing aspect ratio (12.1, 4.6 and 1.1), and increasing mesopore diameter (6.8, 6.8 and 11.4nm), respectively, were synthesized, to understand the role of these structural parameters on enzyme activity and sensor performance. Microscopy and gas sorption experiments confirm that, as required, GOD (~7nm size) immobilizes inside mesopores. Amongst all forms, cuboid SBA-15 showed the highest normalized absolute activity and reusability for at least six cycles. Thus, compared to rod SBA-15, an electrode with GOD immobilized-cuboid SBA-15 showed ten times higher sensitivity of 0.53µA/cm2/mM, with a linearity range of 1.0-7.7mM glucose and a short response time (<5s). Hence, structural features of the host could be directly related to the sensor performance. Therefore, in general, SBA-15 hosts with a smaller particle aspect ratio and a larger internal mesopore diameter (like cuboid), together with appropriate enzyme loading density and presence of ferrocene as an electron mediator, can ensure a superior biosensor performance. Springer Science+Business Media New York, 2015 Mesoporous silica nationallicence Glucose oxidase nationallicence Enzyme immobilization nationallicence Enzyme activity nationallicence Biosensor nationallicence Khan Anees Department of Chemical Engineering, Indian Institute of Technology Bombay, 400076, Powai, Mumbai, India aut Noronha Santosh Department of Chemical Engineering, Indian Institute of Technology Bombay, 400076, Powai, Mumbai, India aut Bandyopadhyaya Rajdip Department of Chemical Engineering, Indian Institute of Technology Bombay, 400076, Powai, Mumbai, India aut Journal of Porous Materials Springer US; http://www.springer-ny.com 22/2(2015-04-01), 369-378 1380-2224 22:2<369 2015 22 10934 https://doi.org/10.1007/s10934-014-9905-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10934-014-9905-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khan Anees Department of Chemical Engineering, Indian Institute of Technology Bombay, 400076, Powai, Mumbai, India aut NATIONALLICENCE 700 1- Noronha Santosh Department of Chemical Engineering, Indian Institute of Technology Bombay, 400076, Powai, Mumbai, India aut NATIONALLICENCE 700 1- Bandyopadhyaya Rajdip Department of Chemical Engineering, Indian Institute of Technology Bombay, 400076, Powai, Mumbai, India aut NATIONALLICENCE 773 0- Journal of Porous Materials Springer US; http://www.springer-ny.com 22/2(2015-04-01), 369-378 1380-2224 22:2<369 2015 22 10934