caa a22 4500 606199764 CHVBK 20210128100940.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10653-014-9630-7 doi (NATIONALLICENCE)springer-10.1007/s10653-014-9630-7 Can the use of medical muds cause genotoxicity in eukaryotic cells? A trial using comet assay [Elektronische Daten] [Gellért Gerencsér, Katalin Szendi, Károly Berényi, Csaba Varga] Despite the lack of knowledge of their exact effects, peloids (natural muds) are widely applied in clinical treatment and prevention of different diseases, especially in rheumatic and gynecological disorders or skin diseases. Primarily we have information on their inorganic components, but only limited data are available on the organic components and nothing on their mechanism of chemical action. The objective of the present study was to detect the DNA-damaging effects (possible genotoxic effect) of peloid samples using the single-cell comet assay on Long Evans rat lymphocytes, human lymphocytes, and Eisenia fetida coelomocytes. Rat and human lymphocytes were exposed to the in toto peloid samples, in vitro. The Eisenia cells were extracted from the coelom of animals kept in the intact peloid sample. An indicator derived from the DNA fluorescence intensity was used in the statistical evaluation. The predominantly organic (Hévíz) sample showed a significant alteration from the negative control in several cases, while the inorganic (Kolop) applied did not. A higher quantity of organic compounds may have an important role in the emergence of DNA damage. The results revealed that medical muds have not only positive health effects but can also contain substances with potential human toxicity risk. Our research provides essential steps towards the creation of a toxicity profile and the possible safe use of peloids as medicinal therapy. Springer Science+Business Media Dordrecht, 2014 Coelomocyte nationallicence Comet assay nationallicence Genotoxicology nationallicence Lymphocytes nationallicence Medicinal muds nationallicence Gerencsér Gellért Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut Szendi Katalin Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut Berényi Károly Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut Varga Csaba Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut Environmental Geochemistry and Health Springer Netherlands 37/1(2015-02-01), 63-70 0269-4042 37:1<63 2015 37 10653 https://doi.org/10.1007/s10653-014-9630-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10653-014-9630-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gerencsér Gellért Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut NATIONALLICENCE 700 1- Szendi Katalin Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut NATIONALLICENCE 700 1- Berényi Károly Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut NATIONALLICENCE 700 1- Varga Csaba Department of Environmental Health, Institute of Public Health Medicine, University of Pécs, Szigeti Str. 12, 7624, Pecs, Hungary aut NATIONALLICENCE 773 0- Environmental Geochemistry and Health Springer Netherlands 37/1(2015-02-01), 63-70 0269-4042 37:1<63 2015 37 10653