caa a22 4500 606201076 CHVBK 20210128100946.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00429-013-0658-8 doi (NATIONALLICENCE)springer-10.1007/s00429-013-0658-8 Anatomical and electrophysiological changes in striatal TH interneurons after loss of the nigrostriatal dopaminergic pathway [Elektronische Daten] [Bengi Ünal, Fulva Shah, Janish Kothari, James Tepper] Using transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of the tyrosine hydroxylase (TH) promoter, we have previously shown that there are approximately 3,000 striatal EGFP-TH interneurons per hemisphere in mice. Here, we report that striatal TH-EGFP interneurons exhibit a small, transient but significant increase in number after unilateral destruction of the nigrostriatal dopaminergic pathway. The increase in cell number is accompanied by electrophysiological and morphological changes. The intrinsic electrophysiological properties of EGFP-TH interneurons ipsilateral to 6-OHDA lesion were similar to those originally reported in intact mice except for a significant reduction in the duration of a characteristic depolarization induced plateau potential. There was a significant change in the distribution of the four previously described electrophysiologically distinct subtypes of striatal TH interneurons. There was a concomitant increase in the frequency of both spontaneous excitatory and inhibitory post-synaptic currents, while their amplitudes did not change. Nigrostriatal lesions did not affect somatic size or dendritic length or branching, but resulted in an increase in the density of proximal dendritic spines and spine-like appendages in EGFP-TH interneurons. The changes indicate that electrophysiology properties and morphology of striatal EGFP-TH interneurons depend on endogenous levels of dopamine arising from the nigrostriatal pathway. Furthermore, these changes may serve to help compensate for the changes in activity of spiny projection neurons that occur following loss of the nigrostriatal innervation in experimental or in early idiopathic Parkinson's disease by increasing feedforward GABAergic inhibition exerted by these interneurons. Springer-Verlag Berlin Heidelberg, 2013 Neostriatum nationallicence Striatal interneurons nationallicence GABA nationallicence 6-OHDA nationallicence Nigrostriatal lesion nationallicence In vitro electrophysiology nationallicence Immunocytochemistry nationallicence Ünal Bengi Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut Shah Fulva Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut Kothari Janish Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut Tepper James Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut Brain Structure and Function Springer Berlin Heidelberg 220/1(2015-01-01), 331-349 1863-2653 220:1<331 2015 220 429 https://doi.org/10.1007/s00429-013-0658-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-013-0658-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ünal Bengi Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut NATIONALLICENCE 700 1- Shah Fulva Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut NATIONALLICENCE 700 1- Kothari Janish Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut NATIONALLICENCE 700 1- Tepper James Center for Molecular and Behavioral Neuroscience, Aidekman Research Center, Rutgers University, 197 University Avenue, 07102, Newark, NJ, USA aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/1(2015-01-01), 331-349 1863-2653 220:1<331 2015 220 429