caa a22 4500 606201475 CHVBK 20210128100948.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00429-013-0667-7 doi (NATIONALLICENCE)springer-10.1007/s00429-013-0667-7 Enhanced characterization of the zebrafish brain as revealed by super-resolution track-density imaging [Elektronische Daten] [Jeremy Ullmann, Fernando Calamante, Shaun Collin, David Reutens, Nyoman Kurniawan] In this study, we explored the use of super-resolution track-density imaging (TDI) for neuroanatomical characterization of the adult zebrafish brain. We compared the quality of image contrast and resolution obtained with T2* magnetic resonance imaging (MRI), diffusion tensor-based imaging (DTI), TDI, and histology. The anatomical structures visualized in 5μm TDI maps corresponded with histology. Moreover, the super-resolution property and the local-directional information provided by directionally encoded color TDI facilitated delineation of a larger number of brain regions, commissures and small white matter tracks when compared to conventional MRI and DTI. In total, we were able to visualize 17 structures that were previously unidentifiable using MR microimaging, such as the four layers of the optic tectum. This study demonstrates the use of TDI for characterization of the adult zebrafish brain as a pivotal tool for future phenotypic examination of transgenic models of neurological diseases. Springer-Verlag Berlin Heidelberg, 2013 Zebrafish nationallicence Brain nationallicence Magnetic resonance nationallicence Diffusion-weighted imaging nationallicence Track-density imaging nationallicence Probabilistic tractography nationallicence 3D GE : Three-dimensional gradient echo nationallicence 3D PLI : Three-dimensional polarized light imaging nationallicence AC : Anterior cerebellar tract nationallicence Cans : Ansulate commissure nationallicence CantD : Anterior commissure, dorsal part nationallicence CantV : Anterior commissure, ventral part nationallicence CC : Cerebellar crest nationallicence CCeG : Cerebellar corpus, granular layer nationallicence CCeM : Cerebellar corpus, molecular layer nationallicence Ccer : Cerebellar commissure nationallicence CON : Caudal octavolateralis nucleus nationallicence Cpop : Postoptic commissure nationallicence Cpost : Posterior commissure nationallicence CSD : Constrained spherical deconvolution nationallicence Cven : Ventral rhombencephalic commissure nationallicence CZ : Central zone of the optic tectum nationallicence D : Dorsal telencephalon nationallicence Dc : Central zone of the dorsal telencephalon nationallicence Dd : Dorsal zone of the dorsal telencephalon nationallicence DEC : Directionally encoded color nationallicence Dl : Lateral zone of the dorsal telencephalon nationallicence Dm : Medial zone of the dorsal telencephalon nationallicence DOT : Dorsomedial optic tract nationallicence Dp : Posterior zone of the dorsal telencephalon nationallicence DTI : Diffusion tensor imaging nationallicence DWI : Diffusion-weighted imaging nationallicence DWZ : Deep white zone of the optic tectum nationallicence FA : Fractional anisotropy nationallicence FOD : Fiber orientation distribution nationallicence FOV : Field of view nationallicence FR : Habenula-interpeduncular tract nationallicence GL : Glomerular layer nationallicence HARDI : High angular-resolution diffusion-weighted imaging nationallicence III : Oculomotor nerve nationallicence IO : Inferior olive nationallicence LCa : Caudal lobe of the cerebellum nationallicence LFB : Lateral forebrain bundle nationallicence LLF : Lateral longitudinal fascicle nationallicence LOT : Lateral olfactory tract nationallicence LVII : Facial lobe nationallicence LX : Vagal lobe nationallicence MA : Mauthner axon nationallicence MFB : Medial forebrain bundle nationallicence MLF : Medial longitudinal fascicle nationallicence MOT : Medial olfactory tract nationallicence MRI : Magnetic resonance imaging nationallicence NEX : Number of averages nationallicence NIN : Interpeduncular nucleus nationallicence nMLF : Nucleus of the medial longitudinal fascicle nationallicence OlfN : Olfactory nerve nationallicence ON : Optic nerve nationallicence PGZ : Periventricular grey zone of the optic tectum nationallicence POF : Primary olfactory layer nationallicence PPa : Parvocellular preoptic nucleus nationallicence RF : Radio frequency nationallicence SNR : Signal-to-noise ratio nationallicence SWGZ : Superficial grey and white zone of the optic tectum nationallicence TDI : Track-density imaging nationallicence TE : Echo time nationallicence TeO : Optic tectum nationallicence TL : Longitudinal torus nationallicence TMCA : Anterior mesencephalon-cerebellar tract nationallicence TPM : Pretecto-mammillary tract nationallicence TR : Repetition time nationallicence TTB : Tractus tectobulbaris nationallicence TTBc : Crossed tecto-bulbar tract nationallicence Val : Lateral division of the valvula cerebelli nationallicence Vas : Vascular lacuna of area postrema nationallicence Ullmann Jeremy Centre for Advanced Imaging, The University of Queensland, 4072, Brisbane, QLD, Australia aut Calamante Fernando Florey Institute of Neuroscience and Mental Health, 3084, Melbourne, VIC, Australia aut Collin Shaun School of Biomedical Sciences, The University of Queensland, 4072, Brisbane, QLD, Australia aut Reutens David Centre for Advanced Imaging, The University of Queensland, 4072, Brisbane, QLD, Australia aut Kurniawan Nyoman Centre for Advanced Imaging, The University of Queensland, 4072, Brisbane, QLD, Australia aut Brain Structure and Function Springer Berlin Heidelberg 220/1(2015-01-01), 457-468 1863-2653 220:1<457 2015 220 429 https://doi.org/10.1007/s00429-013-0667-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-013-0667-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ullmann Jeremy Centre for Advanced Imaging, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Calamante Fernando Florey Institute of Neuroscience and Mental Health, 3084, Melbourne, VIC, Australia aut NATIONALLICENCE 700 1- Collin Shaun School of Biomedical Sciences, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Reutens David Centre for Advanced Imaging, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Kurniawan Nyoman Centre for Advanced Imaging, The University of Queensland, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/1(2015-01-01), 457-468 1863-2653 220:1<457 2015 220 429