caa a22 4500 60620167X CHVBK 20210128100949.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0859-9 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0859-9 Repetitive magnetic stimulation induces plasticity of excitatory postsynapses on proximal dendrites of cultured mouse CA1 pyramidal neurons [Elektronische Daten] [Maximilian Lenz, Steffen Platschek, Viola Priesemann, Denise Becker, Laurent Willems, Ulf Ziemann, Thomas Deller, Florian Müller-Dahlhaus, Peter Jedlicka, Andreas Vlachos] Repetitive transcranial magnetic stimulation (rTMS) of the human brain can lead to long-lasting changes in cortical excitability. However, the cellular and molecular mechanisms which underlie rTMS-induced plasticity remain incompletely understood. Here, we used repetitive magnetic stimulation (rMS) of mouse entorhino-hippocampal slice cultures to study rMS-induced plasticity of excitatory postsynapses. By employing whole-cell patch-clamp recordings of CA1 pyramidal neurons, local electrical stimulations, immunostainings for the glutamate receptor subunit GluA1 and compartmental modeling, we found evidence for a preferential potentiation of excitatory synapses on proximal dendrites of CA1 neurons (2-4h after stimulation). This rMS-induced synaptic potentiation required the activation of voltage-gated sodium channels, L-type voltage-gated calcium channels and N-methyl-d-aspartate-receptors. In view of these findings we propose a cellular model for the preferential strengthening of excitatory synapses on proximal dendrites following rMS in vitro, which is based on a cooperative effect of synaptic glutamatergic transmission and postsynaptic depolarization. Springer-Verlag Berlin Heidelberg, 2014 Hebbian plasticity nationallicence Spike timing dependent plasticity nationallicence Backpropagating action potentials nationallicence AMPA-receptors nationallicence Silent synapses nationallicence Strontium nationallicence Computational modelling nationallicence 3D-reconstruction nationallicence Lenz Maximilian Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Platschek Steffen Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Priesemann Viola Department of Neural Systems and Coding, Max Planck Institute for Brain Research, Frankfurt/Main, Germany aut Becker Denise Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Willems Laurent Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Ziemann Ulf Department of Neurology and Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls-University, Tübingen, Germany aut Deller Thomas Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Müller-Dahlhaus Florian Department of Neurology and Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls-University, Tübingen, Germany aut Jedlicka Peter Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Vlachos Andreas Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3323-3337 1863-2653 220:6<3323 2015 220 429 https://doi.org/10.1007/s00429-014-0859-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0859-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lenz Maximilian Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Platschek Steffen Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Priesemann Viola Department of Neural Systems and Coding, Max Planck Institute for Brain Research, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Becker Denise Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Willems Laurent Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Ziemann Ulf Department of Neurology and Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls-University, Tübingen, Germany aut NATIONALLICENCE 700 1- Deller Thomas Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Müller-Dahlhaus Florian Department of Neurology and Stroke, and Hertie Institute for Clinical Brain Research, Eberhard-Karls-University, Tübingen, Germany aut NATIONALLICENCE 700 1- Jedlicka Peter Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Vlachos Andreas Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Theodor-Stern Kai 7, 60590, Frankfurt/Main, Germany aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3323-3337 1863-2653 220:6<3323 2015 220 429