caa a22 4500 606201785 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0864-z doi (NATIONALLICENCE)springer-10.1007/s00429-014-0864-z Serotonin 5-HT4 receptors and forebrain cholinergic system: receptor expression in identified cell populations [Elektronische Daten] [Raúl Peñas-Cazorla, M. Vilaró] Activation of serotonin 5-HT4 receptors has pro-cognitive effects on memory performance. The proposed underlying neurochemical mechanism is the enhancement of acetylcholine release in frontal cortex and hippocampus elicited by 5-HT4 agonists. Although 5-HT4 receptors are present in brain areas related to cognition, e.g., hippocampus and cortex, the cellular localization of the receptors that might modulate acetylcholine release is unknown at present. We have analyzed, using dual label in situ hybridization, the cellular localization of 5-HT4 receptor mRNA in identified neuronal populations of the rat basal forebrain, which is the source of the cholinergic innervation to cortex and hippocampus. 5-HT4 receptor mRNA was visualized with isotopically labeled oligonucleotide probes, whereas cholinergic, glutamatergic, GABAergic and parvalbumin-synthesizing neurons were identified with digoxigenin-labeled oligonucleotide probes. 5-HT4 receptor mRNA was not detected in the basal forebrain cholinergic cell population. In contrast, basal forebrain GABAergic, parvalbumin synthesizing, and glutamatergic cells contained 5-HT4 receptor mRNA. Hippocampal and cortical glutamatergic neurons also express this receptor. These results indicate that 5-HT4 receptors are not synthesized by cholinergic cells, and thus would be absent from cholinergic terminals. In contrast, several non-cholinergic cell populations within the basal forebrain and its target hippocampal and cortical areas express these receptors and are thus likely to mediate the enhancement of acetylcholine release elicited by 5-HT4 agonists. Springer-Verlag Berlin Heidelberg, 2014 Acetylcholine release nationallicence Septal nuclei nationallicence Nucleus basalis of Meynert nationallicence Basal forebrain GABAergic neurons nationallicence Basal forebrain glutamatergic neurons nationallicence Hippocampus nationallicence Peñas-Cazorla Raúl Department of Neurochemistry and Neuropharmacology, Institut d'Investigacions Biomèdiques de Barcelona (IIBB-CSIC, IDIBAPS), Rosselló, 161 6th Floor, 08036, Barcelona, Spain aut Vilaró M. Department of Neurochemistry and Neuropharmacology, Institut d'Investigacions Biomèdiques de Barcelona (IIBB-CSIC, IDIBAPS), Rosselló, 161 6th Floor, 08036, Barcelona, Spain aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3413-3434 1863-2653 220:6<3413 2015 220 429 https://doi.org/10.1007/s00429-014-0864-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0864-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peñas-Cazorla Raúl Department of Neurochemistry and Neuropharmacology, Institut d'Investigacions Biomèdiques de Barcelona (IIBB-CSIC, IDIBAPS), Rosselló, 161 6th Floor, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Vilaró M. Department of Neurochemistry and Neuropharmacology, Institut d'Investigacions Biomèdiques de Barcelona (IIBB-CSIC, IDIBAPS), Rosselló, 161 6th Floor, 08036, Barcelona, Spain aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3413-3434 1863-2653 220:6<3413 2015 220 429