caa a22 4500 606201793 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0876-8 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0876-8 Stimulation of the subthalamic nucleus engages the cerebellum for motor function in parkinsonian rats [Elektronische Daten] [Alexander Sutton, Katherine O'Connor, Julie Pilitsis, Damian Shin] Deep brain stimulation (DBS) is effective in managing motor symptoms of Parkinson's disease in well-selected individuals. Recently, research has shown that DBS in the basal ganglia (BG) can alter neural circuits beyond the traditional basal ganglia-thalamus-cortical (BG-TH-CX) loop. For instance, functional imaging showed alterations in cerebellar activity with DBS in the subthalamic nucleus (STN). However, these imaging studies revealed very little about how cell-specific cerebellar activity responds to STN stimulation or if these changes contribute to its efficacy. In this study, we assess whether STN-DBS provides efficacy in managing motor symptoms in Parkinson's disease by recruiting cerebellar activity. We do this by applying STN-DBS in hemiparkinsonian rats and simultaneously recording neuronal activity from the STN, brainstem and cerebellum. We found that STN neurons decreased spiking activity by 55% during DBS (P=0.038), which coincided with a decrease in most pedunculopontine tegmental nucleus and Purkinje neurons by 29% (P<0.001) and 28% (P=0.003), respectively. In contrast, spike activity in the deep cerebellar nuclei increased 45% during DBS (P<0.001), which was likely from reduced afferent activity of Purkinje cells. Then, we applied STN-DBS at sub-therapeutic current along with stimulation of the deep cerebellar nuclei and found similar improvement in forelimb akinesia as with therapeutic STN-DBS alone. This suggests that STN-DBS can engage cerebellar activity to improve parkinsonian motor symptoms. Our study is the first to describe how STN-DBS in Parkinson's disease alters cerebellar activity using electrophysiology in vivo and reveal a potential for stimulating the cerebellum to potentiate deep brain stimulation of the subthalamic nucleus. Springer-Verlag Berlin Heidelberg, 2014 6-hydroxydopamine nationallicence Forelimb akinesia nationallicence Parkinson's disease nationallicence Sutton Alexander Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut O'Connor Katherine Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut Pilitsis Julie Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut Shin Damian Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3595-3609 1863-2653 220:6<3595 2015 220 429 https://doi.org/10.1007/s00429-014-0876-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0876-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sutton Alexander Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut NATIONALLICENCE 700 1- O'Connor Katherine Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut NATIONALLICENCE 700 1- Pilitsis Julie Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut NATIONALLICENCE 700 1- Shin Damian Center for Neuropharmacology and Neuroscience, Albany Medical College, 47 New Scotland Ave, 12208, Albany, NY, USA aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3595-3609 1863-2653 220:6<3595 2015 220 429