caa a22 4500 606201858 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0846-1 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0846-1 RhoE deficiency alters postnatal subventricular zone development and the number of calbindin-expressing neurons in the olfactory bulb of mouse [Elektronische Daten] [Begoña Ballester-Lurbe, Susana González-Granero, Enric Mocholí, Enric Poch, María García-Manzanares, Mara Dierssen, Ignacio Pérez-Roger, José García-Verdugo, Rosa Guasch, José Terrado] The subventricular zone represents an important reservoir of progenitor cells in the adult brain. Cells from the subventricular zone migrate along the rostral migratory stream and reach the olfactory bulb, where they originate different types of interneurons. In this work, we have analyzed the role of the small GTPase RhoE/Rnd3 in subventricular zone cell development using mice-lacking RhoE expression. Our results show that RhoE null mice display a remarkable postnatal broadening of the subventricular zone and caudal rostral migratory stream. This broadening was caused by an increase in progenitor proliferation, observed in the second postnatal week but not before, and by an altered migration of the cells, which appeared in disorganized cell arrangements that impaired the appropriate contact between cells in the rostral migratory stream. In addition, the thickness of the granule cell layer in the olfactory bulb was reduced, although the density of granule cells did not differ between wild-type and RhoE null mice. Finally, the lack of RhoE expression affected the olfactory glomeruli inducing a severe reduction of calbindin-expressing interneurons in the periglomerular layer. This was already evident in the newborns and even more pronounced 15days later when RhoE null mice displayed 89% less cells than control mice. Our results indicate that RhoE has pleiotropic functions on subventricular cells because of its role in proliferation and tangential migration, affecting mainly the development of calbindin-expressing cells in the olfactory bulb. Springer-Verlag Berlin Heidelberg, 2014 RhoE nationallicence Rnd proteins nationallicence Subventricular zone nationallicence Rostral migratory stream nationallicence Calbindin nationallicence Proliferation nationallicence Ballester-Lurbe Begoña Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut González-Granero Susana Laboratorio de Neurobiologia Comparada, Instituto Cavanilles de Biodiversidad y Biologia Evolutiva, Universidad de Valencia and CIBER de Enfermedades Neurodegenerativas (CIBERNED), Valencia, Spain aut Mocholí Enric Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut Poch Enric Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut García-Manzanares María Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut Dierssen Mara Systems Biology Program, Center for Genomic Regulation (CRG), Barcelona, Spain aut Pérez-Roger Ignacio Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut García-Verdugo José Laboratorio de Neurobiologia Comparada, Instituto Cavanilles de Biodiversidad y Biologia Evolutiva, Universidad de Valencia and CIBER de Enfermedades Neurodegenerativas (CIBERNED), Valencia, Spain aut Guasch Rosa Laboratorio de Señalización de Proteínas Rho en Neuropatologías, Centro de Investigación Príncipe Felipe, Valencia, Spain aut Terrado José Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3113-3130 1863-2653 220:6<3113 2015 220 429 https://doi.org/10.1007/s00429-014-0846-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0846-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ballester-Lurbe Begoña Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- González-Granero Susana Laboratorio de Neurobiologia Comparada, Instituto Cavanilles de Biodiversidad y Biologia Evolutiva, Universidad de Valencia and CIBER de Enfermedades Neurodegenerativas (CIBERNED), Valencia, Spain aut NATIONALLICENCE 700 1- Mocholí Enric Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- Poch Enric Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- García-Manzanares María Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- Dierssen Mara Systems Biology Program, Center for Genomic Regulation (CRG), Barcelona, Spain aut NATIONALLICENCE 700 1- Pérez-Roger Ignacio Departamento de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut NATIONALLICENCE 700 1- García-Verdugo José Laboratorio de Neurobiologia Comparada, Instituto Cavanilles de Biodiversidad y Biologia Evolutiva, Universidad de Valencia and CIBER de Enfermedades Neurodegenerativas (CIBERNED), Valencia, Spain aut NATIONALLICENCE 700 1- Guasch Rosa Laboratorio de Señalización de Proteínas Rho en Neuropatologías, Centro de Investigación Príncipe Felipe, Valencia, Spain aut NATIONALLICENCE 700 1- Terrado José Instituto de Ciencias Biomédicas, Universidad CEU Cardenal Herrera, Moncada, Valencia, Spain aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3113-3130 1863-2653 220:6<3113 2015 220 429