caa a22 4500 606201882 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0883-9 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0883-9 Evidence for multiple sensory circuits in the brain arising from the respiratory system: an anterograde viral tract tracing study in rodents [Elektronische Daten] [Alice McGovern, Nicholas Davis-Poynter, Seung-Kwon Yang, David Simmons, Michael Farrell, Stuart Mazzone] Complex sensations accompany the activation of sensory neurons within the respiratory system, yet little is known about the organization of sensory pathways in the brain that mediate these sensations. In the present study, we employ anterograde viral neuroanatomical tract tracing with isogenic self-reporting recombinants of HSV-1 strain H129 to map the higher brain regions in receipt of vagal sensory neurons arising from the trachea versus the lungs, and single-cell PCR to characterize the phenotype of sensory neurons arising from these two divisions of the respiratory tree. The results suggest that the upper and lower airways are predominantly innervated by sensory neurons derived from the somatic jugular and visceral nodose cranial ganglia, respectively. This coincides with central circuitry that is predominately somatic-like, arising from the trachea, and visceral-like, arising from the lungs. Although some convergence of sensory pathways was noted in preautonomic cell groups, this was notably absent in thalamic and cortical regions. These data support the notion that distinct afferent subtypes, via distinct central circuits, subserve sensations arising from the upper versus lower airways. The findings may explain why sensations arising from different levels of the respiratory tree are qualitatively and quantitatively unique. Springer-Verlag Berlin Heidelberg, 2014 HSV-1 H129 nationallicence Isogenic recombinant viruses nationallicence Transynaptic nationallicence Somatosensory nationallicence Viscerosensory nationallicence Airway sensations nationallicence McGovern Alice School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut Davis-Poynter Nicholas Clinical Medical Virology Centre/Queensland Children's Medical Research Centre, The University of Queensland and Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, 4029, Brisbane, QLD, Australia aut Yang Seung-Kwon School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut Simmons David School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut Farrell Michael Department of Medical Imaging and Radiation Sciences, Monash University, 3800, Clayton, VIC, Australia aut Mazzone Stuart School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3683-3699 1863-2653 220:6<3683 2015 220 429 https://doi.org/10.1007/s00429-014-0883-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0883-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McGovern Alice School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut NATIONALLICENCE 700 1- Davis-Poynter Nicholas Clinical Medical Virology Centre/Queensland Children's Medical Research Centre, The University of Queensland and Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, 4029, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Yang Seung-Kwon School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut NATIONALLICENCE 700 1- Simmons David School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut NATIONALLICENCE 700 1- Farrell Michael Department of Medical Imaging and Radiation Sciences, Monash University, 3800, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Mazzone Stuart School of Biomedical Sciences, The University of Queensland, 4072, St Lucia, QLD, Australia aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3683-3699 1863-2653 220:6<3683 2015 220 429