caa a22 4500 606201890 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0867-9 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0867-9 Spatial patterns of neuronal activity in rat cerebral cortex during non-rapid eye movement sleep [Elektronische Daten] [Tim Wanger, Wolfram Wetzel, Henning Scheich, Frank Ohl, Jürgen Goldschmidt] It is commonly assumed that cortical activity in non-rapid eye movement sleep (NREMS) is spatially homogeneous on the mesoscopic scale. This is partly due to the limited observational scope of common metabolic or imaging methods in sleep. We used the recently developed technique of thallium-autometallography (TlAMG) to visualize mesoscopic patterns of activity in the sleeping cortex with single-cell resolution. We intravenously injected rats with the lipophilic chelate complex thallium diethyldithiocarbamate (TlDDC) during spontaneously occurring periods of NREMS and mapped the patterns of neuronal uptake of the potassium (K+) probe thallium (Tl+). Using this method, we show that cortical activity patterns are not spatially homogeneous during discrete 5-min episodes of NREMS in unrestrained rats—rather, they are complex and spatially diverse. Along with a relative predominance of infragranular layer activation, we find pronounced differences in metabolic activity of neighboring neuronal assemblies, an observation which lends support to the emerging paradigm that sleep is a distributed process with regulation on the local scale. The Author(s), 2014 Cortical columns nationallicence Infragranular layers nationallicence Laminar microcircuits nationallicence Slow-wave sleep nationallicence Thallium-autometallography nationallicence Wanger Tim Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut Wetzel Wolfram Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut Scheich Henning Emeritus Group Lifelong Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut Ohl Frank Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut Goldschmidt Jürgen Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3469-3484 1863-2653 220:6<3469 2015 220 429 https://doi.org/10.1007/s00429-014-0867-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0867-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wanger Tim Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut NATIONALLICENCE 700 1- Wetzel Wolfram Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut NATIONALLICENCE 700 1- Scheich Henning Emeritus Group Lifelong Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut NATIONALLICENCE 700 1- Ohl Frank Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut NATIONALLICENCE 700 1- Goldschmidt Jürgen Department Systems Physiology of Learning, Leibniz Institute for Neurobiology (LIN), Brenneckestraße 6, 39118, Magdeburg, Germany aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3469-3484 1863-2653 220:6<3469 2015 220 429