caa a22 4500 606201904 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00429-014-0851-4 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0851-4 Amygdalar neuronal plasticity and the interactions of alcohol, sex, and stress [Elektronische Daten] [T. Retson, J. Hoek, R. Sterling, E. Van Bockstaele] Alcohol abuse and alcoholism are major medical problems affecting both men and women. Previous animal studies reported a difference in c-Fos neuronal activation after chronic alcohol exposure; however, females remain an understudied population. To model chronic alcohol exposure match-pair fed adult male and female rats were administered 14days of a liquid ethanol containing diet. Analysis focused on the central nucleus of the amygdala (CeA), a region integral to stress sensitivity and substance abuse. Immunocytochemical approaches identified cells containing ΔFosB, a marker of sustained neuronal activation, and activity patterns within the CeA were mapped by subdivision and rostral-caudal extent. Significant interactions were present between all groups, with gender differences noted among control groups, and ethanol exposed animals having the greatest number of ΔFosB immunoreactive cells indicating baseline dysregulation. Compared with c-Fos, a marker of recent neuronal activation, male ethanol treated animals had similar activity to controls, indicating a neuronal habituation not seen in females. Next, a cohort of animals were exposed to the forced swim test (FST), and c-Fos was examined in addition to FST behavior. Neuronal activity was increased in ethanol exposed animals compared to controls, and control females compared to males, indicating a potentiated stress response. Further, a population of activated neurons were shown to contain either corticotropin releasing factor or enkephalin. The present data suggest that dysregulation in the CeA neuronal activity may underlie some of the negative sequelae of alcohol abuse, and may, in part, underlie the distinctive response seen between genders to alcohol use. Springer-Verlag Berlin Heidelberg, 2014 Chronic alcohol nationallicence Sex differences nationallicence c-Fos nationallicence ΔFosB nationallicence Central nucleus of the amygdala nationallicence Retson T. Department of Neuroscience, Farber Institute for Neurosciences, Thomas Jefferson University, 900 Walnut Street, Suite 417, 19107, Philadelphia, PA, USA aut Hoek J. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 19107, Philadelphia, PA, USA aut Sterling R. Department of Psychiatry, Thomas Jefferson University, 19107, Philadelphia, PA, USA aut Van Bockstaele E. Department of Pharmacology and Physiology, Drexel University, 19107, Philadelphia, PA, USA aut Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3211-3232 1863-2653 220:6<3211 2015 220 429 https://doi.org/10.1007/s00429-014-0851-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0851-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Retson T. Department of Neuroscience, Farber Institute for Neurosciences, Thomas Jefferson University, 900 Walnut Street, Suite 417, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Hoek J. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Sterling R. Department of Psychiatry, Thomas Jefferson University, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Van Bockstaele E. Department of Pharmacology and Physiology, Drexel University, 19107, Philadelphia, PA, USA aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/6(2015-11-01), 3211-3232 1863-2653 220:6<3211 2015 220 429