caa a22 4500 606201939 CHVBK 20210128100950.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0752-6 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0752-6 Altered glutamate/GABA equilibrium in aged mice cortex influences cortical plasticity [Elektronische Daten] [Monika Liguz-Lecznar, Malgorzata Lehner, Aleksandra Kaliszewska, Renata Zakrzewska, Alicja Sobolewska, Malgorzata Kossut] Age-related molecular changes in the synapse can cause plasticity decline. We found an impairment of experience-dependent cortical plasticity is induced by short lasting sensory conditioning in aged mice. However, extending the training procedure from 3 to 7days triggered plasticity in the aged cortex of the same range as in young mice. Additionally, GABAergic markers (GABA, GAD67, VGAT) in young and aged groups that showed the plastic changes were upregulated. This effect was absent in the aged group with impaired plasticity, while the expression of Vglut1 increased in all trained groups. This may reflect the inefficiency of inhibitory mechanisms in the aging brain used to control increased excitation after training and to shape proper signal to noise ratio, which is essential for appropriate stimuli processing. HPLC analysis showed that the glutamate/GABA ratio was significantly reduced in aged animals due to a significant decrease in glutamate level. We also observed a decreased expression of several presynaptic markers involved in excitatory (vesicular glutamate transporter-vglut2) and inhibitory (glutamic acid decarboxylase-GAD67, vesicular GABA transporter VGAT) transmission in the aged barrel cortex. These changes may weaken the plasticity potential of neurons and impede the experience-dependent reorganization of cortical connections. We suggest that the imbalance toward inhibition resulting from a decrease of glutamate content in the aging cerebral cortex, together with GABAergic system ineffectiveness in upregulating GABA level after sensory training, contributes to the impairment of learning-dependent cortical plasticity. Springer-Verlag Berlin Heidelberg, 2014 Aging nationallicence Excitation nationallicence Inhibition nationallicence Learning nationallicence Plasticity nationallicence Liguz-Lecznar Monika Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut Lehner Malgorzata Institute of Psychiatry and Neurology, 8 Sobieskiego St, 02-957, Warsaw, Poland aut Kaliszewska Aleksandra Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut Zakrzewska Renata Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut Sobolewska Alicja Institute of Psychiatry and Neurology, 8 Sobieskiego St, 02-957, Warsaw, Poland aut Kossut Malgorzata Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1681-1693 1863-2653 220:3<1681 2015 220 429 https://doi.org/10.1007/s00429-014-0752-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0752-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liguz-Lecznar Monika Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Lehner Malgorzata Institute of Psychiatry and Neurology, 8 Sobieskiego St, 02-957, Warsaw, Poland aut NATIONALLICENCE 700 1- Kaliszewska Aleksandra Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Zakrzewska Renata Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut NATIONALLICENCE 700 1- Sobolewska Alicja Institute of Psychiatry and Neurology, 8 Sobieskiego St, 02-957, Warsaw, Poland aut NATIONALLICENCE 700 1- Kossut Malgorzata Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 3 Pasteur St, 02-093, Warsaw, Poland aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1681-1693 1863-2653 220:3<1681 2015 220 429