caa a22 4500 606201971 CHVBK 20210128100951.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0720-1 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0720-1 Gender associated circadian oscillations of the clock genes in rat choroid plexus [Elektronische Daten] [T. Quintela, C. Sousa, F. Patriarca, I. Gonçalves, C. Santos] It is well-documented that circadian rhythms are controlled by the circadian master clock of the mammalian brain, located in the suprachiasmatic nucleus (SCN) of the hypothalamus. The SCN clockwork is a cell autonomous mechanism consisting of a series of interlocked transcriptional/post-translational feedback loops. In turn, the SCN controls the seasonal rhythmicity of various biological processes, in particular the secretion pattern of hormones. Although the effects of gonadal hormones on circadian rhythmicity are clearly established, how the SCN integrates and regulates these hormonal stimuli remains unknown. We have previously found that clock genes are expressed in the choroid plexus (CP). Therefore, we compared the circadian expression of these genes in female and male rat CP. We show that there is a 24-h rhythm in the expression of Per2 and Cry2 in males and females. Bmal1 and Per1 expression also varied along the day, but only in females. Bmal1, Clock and Per1 mRNA did not show any significant differences in the CP of males. Moreover, data from cultured CP cells collected at different timepoints revealed significant circadian rhythms in mRNA abundance of Bmal1, Clock and Per2. In conclusion, our data show that the rat CP expresses all canonical clock genes and that their circadian expression differs between genders suggesting that hormones can regulate circadian rhythmicity in CP. Springer-Verlag Berlin Heidelberg, 2014 Choroid plexus nationallicence Circadian rhythm nationallicence Sex hormones nationallicence Clock genes nationallicence Suprachiasmatic nucleus nationallicence Quintela T. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut Sousa C. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut Patriarca F. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut Gonçalves I. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut Santos C. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1251-1262 1863-2653 220:3<1251 2015 220 429 https://doi.org/10.1007/s00429-014-0720-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0720-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Quintela T. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut NATIONALLICENCE 700 1- Sousa C. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut NATIONALLICENCE 700 1- Patriarca F. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut NATIONALLICENCE 700 1- Gonçalves I. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut NATIONALLICENCE 700 1- Santos C. CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1251-1262 1863-2653 220:3<1251 2015 220 429