caa a22 4500 606202005 CHVBK 20210128100951.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0728-6 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0728-6 Expression patterns of c-Fos early gene and phosphorylated ERK in the rat brain following 1-h immobilization stress: concomitant changes induced in association with stress-related sleep rebound [Elektronische Daten] [Fatemeh Keshavarzy, Chantal Bonnet, Gila Bezhadi, Raymond Cespuglio] An immobilization stress (IS) of 1h applied at the beginning of the dark phase is followed by a sleep rebound. During the restraint, serotonin released by the dorsal raphe nucleus within the arcuate area stimulates the availability of corticotropin-like intermediate lobe peptide (CLIP or ACTH18-39). Three hours after the restraint, CLIP, through its hypnogenic properties, contributes to the sleep rebound that follows the IS. Here, we immunohistochemically evaluated protein expression of the immediate early gene, c-Fos and phosphorylated extracellular signal-regulated kinase (p-ERK) in hypothalamic (preoptic area [POA], paraventricular nucleus [PVN], arcuate nucleus [ARC]) and brain stem (dorsal raphe [DR], locus coeruleus [LC]) nuclei involved in the acute response to stress and the subsequent stress-related sleep rebound (recovery period). Immediately after the 1-h restraint, c-Fos and p-ERK expression increased in all structures studied, particularly in PVN and LC. Three hours later, the number of p-ERK- and c-Fos-positive neurons was reduced in PVN and LC (p<0.001) as well as in DR (p<0.01) compared to control animals. In contrast, both c-Fos and p-ERK expression in POA neurons (p<0.01) and c-Fos expression in ARC neurons (p<0.001) were increased 3h after the IS. The marked activation observed in PVN and LC nucleus immediately after the IS confirms that these structures are clearly reactive to stress. However, the high activity observed in POA and ARC neurons during the recovery period, not described to date, highlights the particular part played by these structures in the stress-related sleep rebound. An unbalance in the above processes may contribute to pathological outcomes, such as anxiety and depression. Springer-Verlag Berlin Heidelberg, 2014 Immobilization stress nationallicence Immunohistochemistry nationallicence p-ERK and c-Fos nationallicence Sleep rebound nationallicence Keshavarzy Fatemeh Functional Neuroanatomy Laboratory, Physiology Department and Neuroscience Research Center, Faculty of Medicine, Shahid Beheshti Medical Sciences University, Evin, Tehran, Iran aut Bonnet Chantal Neuroscience Research Center of Lyon (INSERM U1028, CNRS UMR5292 & UCBLyon1), University of Lyon, 8 Avenue Rockefeller, 69373, Lyon, France aut Bezhadi Gila Functional Neuroanatomy Laboratory, Physiology Department and Neuroscience Research Center, Faculty of Medicine, Shahid Beheshti Medical Sciences University, Evin, Tehran, Iran aut Cespuglio Raymond Neuroscience Research Center of Lyon (INSERM U1028, CNRS UMR5292 & UCBLyon1), University of Lyon, 8 Avenue Rockefeller, 69373, Lyon, France aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1793-1804 1863-2653 220:3<1793 2015 220 429 https://doi.org/10.1007/s00429-014-0728-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0728-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keshavarzy Fatemeh Functional Neuroanatomy Laboratory, Physiology Department and Neuroscience Research Center, Faculty of Medicine, Shahid Beheshti Medical Sciences University, Evin, Tehran, Iran aut NATIONALLICENCE 700 1- Bonnet Chantal Neuroscience Research Center of Lyon (INSERM U1028, CNRS UMR5292 & UCBLyon1), University of Lyon, 8 Avenue Rockefeller, 69373, Lyon, France aut NATIONALLICENCE 700 1- Bezhadi Gila Functional Neuroanatomy Laboratory, Physiology Department and Neuroscience Research Center, Faculty of Medicine, Shahid Beheshti Medical Sciences University, Evin, Tehran, Iran aut NATIONALLICENCE 700 1- Cespuglio Raymond Neuroscience Research Center of Lyon (INSERM U1028, CNRS UMR5292 & UCBLyon1), University of Lyon, 8 Avenue Rockefeller, 69373, Lyon, France aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1793-1804 1863-2653 220:3<1793 2015 220 429