caa a22 4500 60620203X CHVBK 20210128100951.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0730-z doi (NATIONALLICENCE)springer-10.1007/s00429-014-0730-z An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology [Elektronische Daten] [Matthias Haberl, Silvia Viana da Silva, Jason Guest, Melanie Ginger, Alexander Ghanem, Christophe Mulle, Marcel Oberlaender, Karl-Klaus Conzelmann, Andreas Frick] Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ∆G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ∆G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits. The Author(s), 2014 Neuronal morphology nationallicence Connectivity nationallicence Sparse labeling nationallicence Circuit reconstruction nationallicence Neuron-type classification nationallicence Alzheimer's disease nationallicence Haberl Matthias Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France aut Viana da Silva Silvia Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, Bordeaux, France aut Guest Jason Digital Neuroanatomy, Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA aut Ginger Melanie Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France aut Ghanem Alexander Max-von-Pettenkofer Institute and Gene Center of the Ludwig-Maximilians-University Munich, Munich, Germany aut Mulle Christophe Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, Bordeaux, France aut Oberlaender Marcel Digital Neuroanatomy, Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA aut Conzelmann Karl-Klaus Max-von-Pettenkofer Institute and Gene Center of the Ludwig-Maximilians-University Munich, Munich, Germany aut Frick Andreas Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1369-1379 1863-2653 220:3<1369 2015 220 429 https://doi.org/10.1007/s00429-014-0730-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0730-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haberl Matthias Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France aut NATIONALLICENCE 700 1- Viana da Silva Silvia Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, Bordeaux, France aut NATIONALLICENCE 700 1- Guest Jason Digital Neuroanatomy, Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA aut NATIONALLICENCE 700 1- Ginger Melanie Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France aut NATIONALLICENCE 700 1- Ghanem Alexander Max-von-Pettenkofer Institute and Gene Center of the Ludwig-Maximilians-University Munich, Munich, Germany aut NATIONALLICENCE 700 1- Mulle Christophe Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, Bordeaux, France aut NATIONALLICENCE 700 1- Oberlaender Marcel Digital Neuroanatomy, Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA aut NATIONALLICENCE 700 1- Conzelmann Karl-Klaus Max-von-Pettenkofer Institute and Gene Center of the Ludwig-Maximilians-University Munich, Munich, Germany aut NATIONALLICENCE 700 1- Frick Andreas Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1369-1379 1863-2653 220:3<1369 2015 220 429