caa a22 4500 606202129 CHVBK 20210128100951.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0755-3 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0755-3 Structural reorganization of pyramidal neurons in the medial prefrontal cortex of alcohol dependent rats is associated with altered glial plasticity [Elektronische Daten] [Airee Kim, Eva Zamora-Martinez, Scott Edwards, Chitra Mandyam] In rodents, chronic intermittent ethanol vapor exposure (CIE) produces alcohol dependence, alters the activity of pyramidal neurons and decreases the number of glial progenitors in the medial prefrontal cortex (mPFC). Adult male Wistar rats were exposed to CIE and were injected with mitotic markers to label and phenotype proliferating cells to test the hypothesis that CIE produces concurrent alterations in the structure of pyramidal neurons and the cell cycle kinetics and developmental stages of glial progenitors in the mPFC. Medial prefrontal cortical tissue was processed for Golgi-Cox staining, immunohistochemistry and Western blotting analysis. CIE increased dendritic arborization and spine densities within basal and apical dendrites of pyramidal neurons via aberrant reorganization of actin cytoskeleton-associated molecules. CIE concomitantly increased the expression of total NR2B subunits without affecting phosphorylation of NR2B at Tyr-1472 or levels of PSD-95. CIE reduced the length of S-phase of the cell cycle of glial progenitors and reduced proliferation and differentiation of progenitors into bHLH transcription factor Olig2-expressing premyelinating oligodendrocyte progenitor cells (OPCs). CIE also produced a corresponding hyperphosphorylation of Olig2, and reduced expression of myelin basic protein. Our findings demonstrate that CIE-induced alterations in OPCs and myelin-related proteins are associated with profound alterations in the structure of pyramidal neurons. In sum, our results not only provide evidence that alcohol dependence leads to pathological changes in the mPFC, which may in part define a cellular basis for cognitive impairments associated with alcoholism, but also show dependence-associated morphological changes in the PFC at the single neuron level. Springer-Verlag Berlin Heidelberg, 2014 Chronic ethanol nationallicence Vapor-induced dependence nationallicence BrdU nationallicence IdU nationallicence CldU nationallicence Olig2 nationallicence MBP nationallicence Kim Airee Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, 92037, La Jolla, CA, USA aut Zamora-Martinez Eva Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, 92037, La Jolla, CA, USA aut Edwards Scott Department of Physiology, Alcohol and Drug Abuse Center of Excellence, LSU Health Sciences Center, New Orleans, LA, USA aut Mandyam Chitra Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, 92037, La Jolla, CA, USA aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1705-1720 1863-2653 220:3<1705 2015 220 429 https://doi.org/10.1007/s00429-014-0755-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0755-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Airee Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Zamora-Martinez Eva Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Edwards Scott Department of Physiology, Alcohol and Drug Abuse Center of Excellence, LSU Health Sciences Center, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Mandyam Chitra Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, 92037, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1705-1720 1863-2653 220:3<1705 2015 220 429