caa a22 4500 60620217X CHVBK 20210128100951.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0732-x doi (NATIONALLICENCE)springer-10.1007/s00429-014-0732-x Lsamp is implicated in the regulation of emotional and social behavior by use of alternative promoters in the brain [Elektronische Daten] [Mari-Anne Philips, Kersti Lilleväli, Indrek Heinla, Hendrik Luuk, Christian Hundahl, Karina Kongi, Taavi Vanaveski, Triin Tekko, Jürgen Innos, Eero Vasar] Limbic system-associated membrane protein (LSAMP) is a neural cell adhesion molecule involved in neurite formation and outgrowth. The purpose of the present study was to characterize the distribution of alternatively transcribed Lsamp isoforms in the mouse brain and its implications on the regulation of behavior. Limbic system-associated membrane protein 1b transcript was visualized by using a mouse strain expressing beta-galactosidase under the control of Lsamp 1b promoter. The distribution of Lsamp 1a transcript and summarized expression of the Lsamp transcripts was investigated by non-radioactive in situ RNA hybridization analysis. Cross-validation was performed by using radioactive in situ hybridization with oligonucleotide probes. Quantitative RT-PCR was used to study correlations between the expression of Lsamp isoforms and behavioral parameters. The expression pattern of two promoters differs remarkably from the developmental initiation at embryonic day 12.5. Limbic system-associated membrane protein 1a promoter is active in "classic” limbic structures where the hippocampus and amygdaloid area display the highest expression. Promoter 1b is mostly active in the thalamic sensory nuclei and cortical sensory areas, but also in areas that regulate stress and arousal. Higher levels of Lsamp 1a transcript had significant correlations with all of the measures indicating higher trait anxiety in the elevated plus-maze test. Limbic system-associated membrane protein transcript levels in the hippocampus and ventral striatum correlated with behavioral parameters in the social interaction test. The data are in line with decreased anxiety and alterations in social behavior in Lsamp-deficient mice. We propose that Lsamp is involved in emotional and social operating systems by complex regulation of two alternative promoters. The Author(s), 2014 Lsamp nationallicence Alternative promoters nationallicence Limbic system nationallicence Sensory pathways nationallicence Anxiety nationallicence Social behavior nationallicence Philips Mari-Anne Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Lilleväli Kersti Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Heinla Indrek Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Luuk Hendrik Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Hundahl Christian Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Kongi Karina Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Vanaveski Taavi Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Tekko Triin Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Innos Jürgen Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Vasar Eero Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1381-1393 1863-2653 220:3<1381 2015 220 429 https://doi.org/10.1007/s00429-014-0732-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0732-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Philips Mari-Anne Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Lilleväli Kersti Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Heinla Indrek Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Luuk Hendrik Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Hundahl Christian Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Kongi Karina Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Vanaveski Taavi Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Tekko Triin Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Innos Jürgen Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 700 1- Vasar Eero Department of Physiology, Institute of Biomedicine and Translational Medicine, University of Tartu, 50411, Tartu, Estonia aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1381-1393 1863-2653 220:3<1381 2015 220 429