caa a22 4500 60620220X CHVBK 20210128100952.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0734-8 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0734-8 Neck muscle afferents influence oromotor and cardiorespiratory brainstem neural circuits [Elektronische Daten] [I. Edwards, V. Lall, J. Paton, Y. Yanagawa, G. Szabo, S. Deuchars, J. Deuchars] Sensory information arising from the upper neck is important in the reflex control of posture and eye position. It has also been linked to the autonomic control of the cardiovascular and respiratory systems. Whiplash associated disorders (WAD) and cervical dystonia, which involve disturbance to the neck region, can often present with abnormalities to the oromotor, respiratory and cardiovascular systems. We investigated the potential neural pathways underlying such symptoms. Simulating neck afferent activity by electrical stimulation of the second cervical nerve in a working heart brainstem preparation (WHBP) altered the pattern of central respiratory drive and increased perfusion pressure. Tracing central targets of these sensory afferents revealed projections to the intermedius nucleus of the medulla (InM). These anterogradely labelled afferents co-localised with parvalbumin and vesicular glutamate transporter 1 indicating that they are proprioceptive. Anterograde tracing from the InM identified projections to brain regions involved in respiratory, cardiovascular, postural and oro-facial behaviours—the neighbouring hypoglossal nucleus, facial and motor trigeminal nuclei, parabrachial nuclei, rostral and caudal ventrolateral medulla and nucleus ambiguus. In brain slices, electrical stimulation of afferent fibre tracts lateral to the cuneate nucleus monosynaptically excited InM neurones. Direct stimulation of the InM in the WHBP mimicked the response of second cervical nerve stimulation. These results provide evidence of pathways linking upper cervical sensory afferents with CNS areas involved in autonomic and oromotor control, via the InM. Disruption of these neuronal pathways could, therefore, explain the dysphagic and cardiorespiratory abnormalities which may accompany cervical dystonia and WAD. The Author(s), 2014 Proprioception nationallicence Autonomic nationallicence Immunohistochemistry nationallicence Electrophysiology nationallicence Edwards I. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Lall V. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Paton J. School of Physiology and Pharmacology, Bristol Heart Institute, University of Bristol, Medical Sciences Building, Bristol, BS8 1TD, UK aut Yanagawa Y. Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine JST, CREST, 371-8511, Maebashi, Japan aut Szabo G. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, 1450, Budapest, Hungary aut Deuchars S. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Deuchars J. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1421-1436 1863-2653 220:3<1421 2015 220 429 https://doi.org/10.1007/s00429-014-0734-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0734-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Edwards I. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Lall V. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Paton J. School of Physiology and Pharmacology, Bristol Heart Institute, University of Bristol, Medical Sciences Building, Bristol, BS8 1TD, UK aut NATIONALLICENCE 700 1- Yanagawa Y. Department of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine JST, CREST, 371-8511, Maebashi, Japan aut NATIONALLICENCE 700 1- Szabo G. Department of Gene Technology and Developmental Neurobiology, Institute of Experimental Medicine, 1450, Budapest, Hungary aut NATIONALLICENCE 700 1- Deuchars S. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Deuchars J. School of Biomedical Sciences, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1421-1436 1863-2653 220:3<1421 2015 220 429