caa a22 4500 606202250 CHVBK 20210128100952.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0754-4 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0754-4 Spatial normalization of ultrahigh resolution 7T magnetic resonance imaging data of the postmortem human subthalamic nucleus: a multistage approach [Elektronische Daten] [Marcel Weiss, Anneke Alkemade, Max Keuken, Christa Műller-Axt, Stefan Geyer, Robert Turner, Birte Forstmann] In this paper, we describe a novel processing strategy for the spatial normalization of ultrahigh resolution magnetic resonance imaging (MRI) data of small ex vivo samples into MNI standard space. We present a multistage scanning and registration method for data of the subthalamic nucleus (STN) obtained using ultrahigh 7T MRI on four human postmortem brain samples. Four whole brains were obtained and subjected to multistage MRI scanning, corresponding to four different brain dissection stages. Data sets were acquired with an isotropic resolution of 100 μm enabling accurate manual segmentation of the STN. Spatial normalization to MNI reference space was performed, probability maps were calculated, and results were cross-checked with an independent in vivo dataset showing significant overlay. Normalization of results obtained from small tissue samples into MNI standard space will facilitate comparison between individual subjects, as well as between studies. When combining ultrahigh resolution MRI of ex vivo samples with histological studies via blockface imaging, our method enables further insight and inference as multimodal data can be compared within the same reference space. This novel technique may be of value for research purposes using functional MRI techniques, and in the future may be of assistance for anatomical orientation in clinical practice. Springer-Verlag Berlin Heidelberg, 2014 Ultrahigh resolution MRI nationallicence Basal ganglia nationallicence Parkinson's disease nationallicence Subthalamic nucleus nationallicence MR microscopy nationallicence Probability maps nationallicence Weiss Marcel Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut Alkemade Anneke Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut Keuken Max Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut Műller-Axt Christa Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut Geyer Stefan Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany aut Turner Robert Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany aut Forstmann Birte Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1695-1703 1863-2653 220:3<1695 2015 220 429 https://doi.org/10.1007/s00429-014-0754-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0754-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weiss Marcel Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Alkemade Anneke Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Keuken Max Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Műller-Axt Christa Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Geyer Stefan Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany aut NATIONALLICENCE 700 1- Turner Robert Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany aut NATIONALLICENCE 700 1- Forstmann Birte Cognitive Science Center Amsterdam, University of Amsterdam, Nieuwe Achtergracht 129, 1018 WS, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1695-1703 1863-2653 220:3<1695 2015 220 429