caa a22 4500 606202323 CHVBK 20210128100952.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0724-x doi (NATIONALLICENCE)springer-10.1007/s00429-014-0724-x In vivo electroporation to physiologically identified deep brain regions in postnatal mammals [Elektronische Daten] [Nami Ohmura, Kazuha Kawasaki, Takemasa Satoh, Yoshio Hata] Genetic manipulation is widely used to research the central nervous system (CNS). The manipulation of molecular expression in a small number of neurons permits the detailed investigation of the role of specific molecules on the function and morphology of the neurons. Electroporation is a broadly used technique for gene transfer in the CNS. However, the targeting of gene transfer using electroporation in postnatal animals was restricted to the cortex, hippocampus, or the region facing the ventricle in previous reports. Electroporation targeting of deep brain structures, such as the thalamus, has been difficult. We introduce a novel electroporation technique that enables gene transfer to a physiologically identified deep brain region using a glass pipette. We recorded neural activity in young-adult mice to identify the location of the lateral geniculate nucleus (LGN) of the thalamus, using a glass pipette electrode containing the plasmid DNA encoding enhanced green fluorescent protein (EGFP). The location of the LGN was confirmed by monitoring visual responses, and the plasmid solution was pressure-injected into the recording site. Voltage pulses were delivered through the glass pipette electrode. Several EGFP-labeled somata and dendrites were observed in the LGN after a few weeks, and labeled axons were found in the visual cortex. The EGFP-expressing structures were observed in detail sufficient to reconstruct their morphology in three dimensions. We further confirmed the applicability of this technique in cats. This method should be useful for the transfer of various genes into cells in physiologically identified brain regions in rodents and gyrencephalic mammals. Springer-Verlag Berlin Heidelberg, 2014 Electroporation nationallicence Lateral geniculate nucleus nationallicence Visual cortex nationallicence Cat nationallicence Gene transfer nationallicence Ohmura Nami Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences, 86 Nishicho, 683-8503, Yonago, Japan aut Kawasaki Kazuha Division of Neurobiology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, Japan aut Satoh Takemasa Division of Neurobiology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, Japan aut Hata Yoshio Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences, 86 Nishicho, 683-8503, Yonago, Japan aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1307-1316 1863-2653 220:3<1307 2015 220 429 https://doi.org/10.1007/s00429-014-0724-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0724-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohmura Nami Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences, 86 Nishicho, 683-8503, Yonago, Japan aut NATIONALLICENCE 700 1- Kawasaki Kazuha Division of Neurobiology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, Japan aut NATIONALLICENCE 700 1- Satoh Takemasa Division of Neurobiology, School of Life Sciences, Tottori University Faculty of Medicine, Yonago, Japan aut NATIONALLICENCE 700 1- Hata Yoshio Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences, 86 Nishicho, 683-8503, Yonago, Japan aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1307-1316 1863-2653 220:3<1307 2015 220 429