caa a22 4500 606202331 CHVBK 20210128100952.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00429-014-0726-8 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0726-8 A gradual depth-dependent change in connectivity features of supragranular pyramidal cells in rat barrel cortex [Elektronische Daten] [Jochen Staiger, Ingo Bojak, Stéphanie Miceli, Dirk Schubert] Recent experimental evidence suggests a finer genetic, structural and functional subdivision of the layers which form a cortical column. The classical layer II/III (LII/III) of rodent neocortex integrates ascending sensory information with contextual cortical information for behavioral read-out. We systematically investigated to which extent regular-spiking supragranular pyramidal neurons, located at different depths within the cortex, show different input-output connectivity patterns. Combining glutamate uncaging with whole-cell recordings and biocytin filling, we revealed a novel cellular organization of LII/III: (1) "Lower LII/III” pyramidal cells receive a very strong excitatory input from lemniscal LIV and much fewer inputs from paralemniscal LVa. They project to all layers of the home column, including a feedback projection to LIV, whereas transcolumnar projections are relatively sparse. (2) "Upper LII/III” pyramidal cells also receive their strongest input from LIV, but in addition, a very strong and dense excitatory input from LVa. They project extensively to LII/III as well as LVa and Vb of their home and neighboring columns. (3) "Middle LII/III” pyramidal cell shows an intermediate connectivity phenotype that stands in many ways in between the features described for lower versus upper LII/III. "Lower LII/III” intracolumnarly segregates and transcolumnarly integrates lemniscal information, whereas "upper LII/III” seems to integrate lemniscal with paralemniscal information. This suggests a fine-grained functional subdivision of the supragranular compartment containing multiple circuits without any obvious cytoarchitectonic, other structural or functional correlate of a laminar border in rodent barrel cortex. The Author(s), 2014 Cortical microcircuits nationallicence Barrel-related column nationallicence Lemniscal system nationallicence Paralemniscal system nationallicence Caged glutamate nationallicence Staiger Jochen Institute for Neuroanatomy, University Medicine Göttingen, Kreuzbergring 36, 37075, Göttingen, Germany aut Bojak Ingo School of Systems Engineering, University of Reading, Whiteknights, PO Box 225, RG6 6AY, Reading, Berkshire, UK aut Miceli Stéphanie Donders Institute for Brain, Cognition and Behavior, Centre for Neuroscience, Radboud University Nijmegen Medical Centre, POB 9101//126, 6500 HB, Nijmegen, The Netherlands aut Schubert Dirk Donders Institute for Brain, Cognition and Behavior, Centre for Neuroscience, Radboud University Nijmegen Medical Centre, POB 9101//126, 6500 HB, Nijmegen, The Netherlands aut Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1317-1337 1863-2653 220:3<1317 2015 220 429 https://doi.org/10.1007/s00429-014-0726-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0726-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Staiger Jochen Institute for Neuroanatomy, University Medicine Göttingen, Kreuzbergring 36, 37075, Göttingen, Germany aut NATIONALLICENCE 700 1- Bojak Ingo School of Systems Engineering, University of Reading, Whiteknights, PO Box 225, RG6 6AY, Reading, Berkshire, UK aut NATIONALLICENCE 700 1- Miceli Stéphanie Donders Institute for Brain, Cognition and Behavior, Centre for Neuroscience, Radboud University Nijmegen Medical Centre, POB 9101//126, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Schubert Dirk Donders Institute for Brain, Cognition and Behavior, Centre for Neuroscience, Radboud University Nijmegen Medical Centre, POB 9101//126, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/3(2015-05-01), 1317-1337 1863-2653 220:3<1317 2015 220 429