caa a22 4500 606202390 CHVBK 20210128100952.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0787-8 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0787-8 Development of the blood-brain barrier within the paraventricular nucleus of the hypothalamus: influence of fetal glucocorticoid excess [Elektronische Daten] [Krystle Frahm, Stuart Tobet] The blood-brain barrier (BBB) is a critical contributor to brain function. To understand its development and potential function in different brain regions, the postnatal (P) BBB was investigated in the mouse cortex (CTX), lateral hypothalamus, and paraventricular nucleus of the hypothalamus (PVN). Brains were examined on postnatal days (P)12, P22 and P52 for BBB competency and for pericytes as key cellular components of the BBB demarcated by immunoreactive desmin. Glucocorticoid influences (excess dexamethasone; dex) during prenatal development were also assessed for their impact on the blood vessels within these regions postnatally. At P12, there was significantly more extravascular leakage of a low molecular weight dye (fluorescein isothiocyanate) in the CTX than within hypothalamic regions. For pericytes, there were low levels of desmin immunoreactivity at P12 that increased with age for all regions. There was more desmin immunoreactivity present in the PVN at each age examined. Fetal dex exposure resulted in decreased blood vessel density within the PVN at P20. In the CTX, dex exposure increased BBB competency, in contrast to the PVN where there was a decrease in BBB competency and increased pericyte presence. Overall, unique alterations in the functioning of the BBB within the PVN may provide a novel mechanism for fetal antecedent programming that may influence adult disorders. The Author(s), 2014 Blood-brain barrier nationallicence Prenatal stress nationallicence Pericytes nationallicence Paraventricular nucleus of the hypothalamus nationallicence Frahm Krystle Program in Cell and Molecular Biology, Colorado State University, 1617 Campus Delivery, 80523-1617, Fort Collins, CO, USA aut Tobet Stuart Program in Cell and Molecular Biology, Colorado State University, 1617 Campus Delivery, 80523-1617, Fort Collins, CO, USA aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2225-2234 1863-2653 220:4<2225 2015 220 429 https://doi.org/10.1007/s00429-014-0787-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0787-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frahm Krystle Program in Cell and Molecular Biology, Colorado State University, 1617 Campus Delivery, 80523-1617, Fort Collins, CO, USA aut NATIONALLICENCE 700 1- Tobet Stuart Program in Cell and Molecular Biology, Colorado State University, 1617 Campus Delivery, 80523-1617, Fort Collins, CO, USA aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2225-2234 1863-2653 220:4<2225 2015 220 429