caa a22 4500 606202447 CHVBK 20210128100953.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0770-4 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0770-4 White matter tracts for the trafficking of neural progenitor cells characterized by cellular MRI and immunohistology: the role of CXCL12/CXCR4 signaling [Elektronische Daten] [Chiao-Chi Chen, Yi-Hua Hsu, D. Jayaseema, Jeou-Yuan Chen, Dueng-Yuan Hueng, Chen Chang] White matter tracts are important for the trafficking of neural progenitor cells (NPCs) in both normal and pathological conditions, but the underlying mechanism is not clear. The directionality of white matter is advantageous for molecules or cells to distribute over a long distance, but this feature is unlikely solely responsible for efficient migration. The present study hypothesizes that the efficient migration of NPCs into white matter is under the influences of neurochemical attraction—CXCL12/CXCR4 signaling, a major mechanism underlying the targeted migration of NPCs. To test this view, the present study investigated the effects of CXCL12 administration into the corpus callosum (CC) on the migratory behavior of transplanted NPCs. A living animal tracking platform based on MRI and a magnetic cell labeling technique was employed. The NPCs were magnetically labeled and then transplanted at the right end of the CC. CXCL12 was infused continuously at the left end. Migration of NPCs was monitored repeatedly over a 7-day course using 3D gradient echo T2*-weighted imaging. It was found that, CXCL12 induced NPCs to migrate up to 1,881μm from the graft whereas the spontaneous migration was mere 200μm. CXCL12 induced migration that was nine times as efficient in the speed. The results indicate that the CXCL12/CXCR4 signaling may be a mechanism via which NPCs efficiently migrate along the white matter tracts. The study also presents a potential strategy for facilitating the targeted migration in NPC therapy for brain disorders. The Author(s), 2014 SDF nationallicence CXCR4 nationallicence Stem cell nationallicence Migration nationallicence Tropism nationallicence White matter nationallicence Neurogenesis nationallicence Homing nationallicence Directed migration nationallicence NPCs : Neural progenitor cells nationallicence CC : Corpus callosum nationallicence MPIO : Micron-sized paramagnetic iron oxide nationallicence 3D : Three dimensional nationallicence T2*WI : T2*weighted imaging nationallicence PB : Prussian blue nationallicence APC : Allophycocyanin nationallicence 7-AAD : 7-Aminoactinomycin-D nationallicence NDS : Normal donkey serum nationallicence DAB : Diaminobenzidine nationallicence Chen Chiao-Chi N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut Hsu Yi-Hua N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut Jayaseema D. N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut Chen Jeou-Yuan N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut Hueng Dueng-Yuan Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, 325, Section 2, Cheng-Kung Road, Neihu, 11490, Taipei, Taiwan aut Chang Chen N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2073-2085 1863-2653 220:4<2073 2015 220 429 https://doi.org/10.1007/s00429-014-0770-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0770-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Chiao-Chi N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Hsu Yi-Hua N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Jayaseema D. N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chen Jeou-Yuan N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut NATIONALLICENCE 700 1- Hueng Dueng-Yuan Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, 325, Section 2, Cheng-Kung Road, Neihu, 11490, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chang Chen N123, Institute of Biomedical Sciences, Academia Sinica, 128, Section2, Academia Road, Nankang, 11529, Taipei, Taiwan aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2073-2085 1863-2653 220:4<2073 2015 220 429