caa a22 4500 606202463 CHVBK 20210128100953.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0775-z doi (NATIONALLICENCE)springer-10.1007/s00429-014-0775-z Postnatal development of the molecular complex underlying astrocyte polarization [Elektronische Daten] [Lisa Lunde, Laura Camassa, Eystein Hoddevik, Faraz Khan, Ole Ottersen, Henning Boldt, Mahmood Amiry-Moghaddam] Astrocytes are highly polarised cells with processes that ensheath microvessels, cover the brain surface, and abut synapses. The endfoot membrane domains facing microvessels and pia are enriched with aquaporin-4 water channels (AQP4) and other members of the dystrophin associated protein complex (DAPC). Several lines of evidence show that loss of astrocyte polarization, defined by the loss of proteins that are normally enriched in astrocyte endfeet, is a common denominator of several neurological diseases such as mesial temporal lobe epilepsy, Alzheimer's disease, and stroke. Little is known about the mechanisms responsible for inducing astrocyte polarization in vivo. Here we introduce the term endfoot-basal lamina junctional complex (EBJC) to denote the proteins that consolidate and characterize the gliovascular interface. The present study was initiated in order to resolve the developmental profile of the EBJC in mouse brain. We show that the EBJC is established after the first week postnatally. Through a combination of methodological approaches, including light microscopic and high resolution immunogold cytochemistry, quantitative RT-PCR, and Western blotting, we demonstrate that the different members of this complex exhibit distinct ontogenic profiles--with the extracellular matrix (ECM) proteins laminin and agrin appearing earlier than the other members of the complex. Specifically, while laminin and agrin expression peak at P7, quantitative immunoblot analyses indicate that AQP4, α-syntrophin, and the inwardly rectifying K+channel Kir4.1 expression increases towards adulthood. Our findings are consistent with ECM having an instructive role in establishing astrocyte polarization in postnatal development and emphasize the need to explore the involvement of ECM in neurological disease. The Author(s), 2014 Astrocyte polarization nationallicence Aquaporin-4 nationallicence Extracellular matrix nationallicence Dystrophin associated protein complex nationallicence Brain development nationallicence Immunogold cytochemistry nationallicence Lunde Lisa Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Camassa Laura Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Hoddevik Eystein Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Khan Faraz Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Ottersen Ole Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Boldt Henning Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Amiry-Moghaddam Mahmood Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2087-2101 1863-2653 220:4<2087 2015 220 429 https://doi.org/10.1007/s00429-014-0775-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0775-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lunde Lisa Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 700 1- Camassa Laura Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 700 1- Hoddevik Eystein Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 700 1- Khan Faraz Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 700 1- Ottersen Ole Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 700 1- Boldt Henning Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 700 1- Amiry-Moghaddam Mahmood Laboratory of Molecular Neuroscience, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2087-2101 1863-2653 220:4<2087 2015 220 429