caa a22 4500 60620248X CHVBK 20210128100953.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0774-0 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0774-0 Whole-brain mapping of behaviourally induced neural activation in mice [Elektronische Daten] [Dulcie Vousden, Jonathan Epp, Hiroyuki Okuno, Brian Nieman, Matthijs van Eede, Jun Dazai, Timothy Ragan, Haruhiko Bito, Paul Frankland, Jason Lerch, R. Henkelman] The ability to visualize behaviourally evoked neural activity patterns across the rodent brain is essential for understanding the distributed brain networks mediating particular behaviours. However, current imaging methods are limited in their spatial resolution and/or ability to obtain brain-wide coverage of functional activity. Here, we describe a new automated method for obtaining cellular-level, whole-brain maps of behaviourally induced neural activity in the mouse. This method combines the use of transgenic immediate-early gene reporter mice to visualize neural activity; serial two-photon tomography to image the entire brain at cellular resolution; advanced image processing algorithms to count the activated neurons and align the datasets to the Allen Mouse Brain Atlas; and statistical analysis to identify the network of activated brain regions evoked by behaviour. We demonstrate the use of this approach to determine the whole-brain networks activated during the retrieval of fear memories. Consistent with previous studies, we identified a large network of amygdalar, hippocampal, and neocortical brain regions implicated in fear memory retrieval. Our proposed methods can thus be used to map cellular networks involved in the expression of normal behaviours as well as to investigate in depth circuit dysfunction in mouse models of neurobiological disease. Springer-Verlag Berlin Heidelberg, 2014 Neuroimaging nationallicence Immediate-early genes nationallicence Memory nationallicence Functional networks nationallicence Serial two-photon tomography nationallicence Vousden Dulcie Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut Epp Jonathan Program in Neuroscience and Mental Health, The Hospital for Sick Children, 555 University Ave., ON M5G 1X8, Toronto, Canada aut Okuno Hiroyuki Department of Neurochemistry, The University of Tokyo Graduate School of Medicine, CREST-JST, Bunkyo-ku, Hongo, 113-0033, Tokyo, Japan aut Nieman Brian Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut van Eede Matthijs Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut Dazai Jun Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut Ragan Timothy TissueVision, Inc., 955 Massachusetts Ave Ste 316, 02139, Cambridge, MA, USA aut Bito Haruhiko Department of Neurochemistry, The University of Tokyo Graduate School of Medicine, CREST-JST, Bunkyo-ku, Hongo, 113-0033, Tokyo, Japan aut Frankland Paul Program in Neuroscience and Mental Health, The Hospital for Sick Children, 555 University Ave., ON M5G 1X8, Toronto, Canada aut Lerch Jason Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut Henkelman R. Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2043-2057 1863-2653 220:4<2043 2015 220 429 https://doi.org/10.1007/s00429-014-0774-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0774-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vousden Dulcie Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut NATIONALLICENCE 700 1- Epp Jonathan Program in Neuroscience and Mental Health, The Hospital for Sick Children, 555 University Ave., ON M5G 1X8, Toronto, Canada aut NATIONALLICENCE 700 1- Okuno Hiroyuki Department of Neurochemistry, The University of Tokyo Graduate School of Medicine, CREST-JST, Bunkyo-ku, Hongo, 113-0033, Tokyo, Japan aut NATIONALLICENCE 700 1- Nieman Brian Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut NATIONALLICENCE 700 1- van Eede Matthijs Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut NATIONALLICENCE 700 1- Dazai Jun Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut NATIONALLICENCE 700 1- Ragan Timothy TissueVision, Inc., 955 Massachusetts Ave Ste 316, 02139, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Bito Haruhiko Department of Neurochemistry, The University of Tokyo Graduate School of Medicine, CREST-JST, Bunkyo-ku, Hongo, 113-0033, Tokyo, Japan aut NATIONALLICENCE 700 1- Frankland Paul Program in Neuroscience and Mental Health, The Hospital for Sick Children, 555 University Ave., ON M5G 1X8, Toronto, Canada aut NATIONALLICENCE 700 1- Lerch Jason Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut NATIONALLICENCE 700 1- Henkelman R. Mouse Imaging Centre, The Hospital for Sick Children, 25 Orde St., ON M5T 3H7, Toronto, Canada aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2043-2057 1863-2653 220:4<2043 2015 220 429