caa a22 4500 606202617 CHVBK 20210128100954.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0800-2 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0800-2 Reorganization of supramammillary-hippocampal pathways in the rat pilocarpine model of temporal lobe epilepsy: evidence for axon terminal sprouting [Elektronische Daten] [Rabia Soussi, Jean-Luc Boulland, Emilie Bassot, Hélène Bras, Patrice Coulon, Farrukh Chaudhry, Jon Storm-Mathisen, Lotfi Ferhat, Monique Esclapez] In mesial temporal lobe epilepsy (MTLE), spontaneous seizures likely originate from a multi-structural epileptogenic zone, including several regions of the limbic system connected to the hippocampal formation. In this study, we investigate the structural connectivity between the supramammillary nucleus (SuM) and the dentate gyrus (DG) in the model of MTLE induced by pilocarpine in the rat. This hypothalamic nucleus, which provides major extracortical projections to the hippocampal formation, plays a key role in the regulation of several hippocampus-dependent activities, including theta rhythms, memory function and emotional behavior, such as stress and anxiety, functions that are known to be altered in MTLE. Our findings demonstrate a marked reorganization of DG afferents originating from the SuM in pilocarpine-treated rats. This reorganization, which starts during the latent period, is massive when animals become epileptic and continue to evolve during epilepsy. It is characterized by an aberrant distribution and an increased number of axon terminals from neurons of both lateral and medial regions of the SuM, invading the entire inner molecular layer of the DG. This reorganization, which reflects an axon terminal sprouting from SuM neurons, could contribute to trigger spontaneous seizures within an altered hippocampal intrinsic circuitry. The Author(s), 2014 Hippocampus nationallicence Dentate gyrus nationallicence GAD nationallicence VGAT nationallicence VGLUT2 nationallicence Hypothalamus nationallicence SuM nationallicence Soussi Rabia INSERM, UMR 1106, Institut de Neurosciences des Systèmes - INS, 13385, Marseille, France aut Boulland Jean-Luc Biotechnology Center, Institute of Basic Medical Sciences and Center for Molecular Biology and Neuroscience, University of Oslo, 0317, Oslo, Norway aut Bassot Emilie INSERM, UMR 1106, Institut de Neurosciences des Systèmes - INS, 13385, Marseille, France aut Bras Hélène Aix-Marseille University, 13385, Marseille, France aut Coulon Patrice Aix-Marseille University, 13385, Marseille, France aut Chaudhry Farrukh Biotechnology Center, Institute of Basic Medical Sciences and Center for Molecular Biology and Neuroscience, University of Oslo, 0317, Oslo, Norway aut Storm-Mathisen Jon Department of Anatomy, Institute of Basic Medical Sciences and Center for Molecular Biology and Neuroscience, University of Oslo, 0317, Oslo, Norway aut Ferhat Lotfi Aix-Marseille University, 13385, Marseille, France aut Esclapez Monique INSERM, UMR 1106, Institut de Neurosciences des Systèmes - INS, 13385, Marseille, France aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2449-2468 1863-2653 220:4<2449 2015 220 429 https://doi.org/10.1007/s00429-014-0800-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0800-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Soussi Rabia INSERM, UMR 1106, Institut de Neurosciences des Systèmes - INS, 13385, Marseille, France aut NATIONALLICENCE 700 1- Boulland Jean-Luc Biotechnology Center, Institute of Basic Medical Sciences and Center for Molecular Biology and Neuroscience, University of Oslo, 0317, Oslo, Norway aut NATIONALLICENCE 700 1- Bassot Emilie INSERM, UMR 1106, Institut de Neurosciences des Systèmes - INS, 13385, Marseille, France aut NATIONALLICENCE 700 1- Bras Hélène Aix-Marseille University, 13385, Marseille, France aut NATIONALLICENCE 700 1- Coulon Patrice Aix-Marseille University, 13385, Marseille, France aut NATIONALLICENCE 700 1- Chaudhry Farrukh Biotechnology Center, Institute of Basic Medical Sciences and Center for Molecular Biology and Neuroscience, University of Oslo, 0317, Oslo, Norway aut NATIONALLICENCE 700 1- Storm-Mathisen Jon Department of Anatomy, Institute of Basic Medical Sciences and Center for Molecular Biology and Neuroscience, University of Oslo, 0317, Oslo, Norway aut NATIONALLICENCE 700 1- Ferhat Lotfi Aix-Marseille University, 13385, Marseille, France aut NATIONALLICENCE 700 1- Esclapez Monique INSERM, UMR 1106, Institut de Neurosciences des Systèmes - INS, 13385, Marseille, France aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2449-2468 1863-2653 220:4<2449 2015 220 429