caa a22 4500 606202641 CHVBK 20210128100954.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0780-2 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0780-2 Dendritic planarity of Purkinje cells is independent of Reelin signaling [Elektronische Daten] [Jinkyung Kim, Tae-Ju Park, Namseop Kwon, Dongmyeong Lee, Seunghwan Kim, Yoshiki Kohmura, Tetsuya Ishikawa, Kyong-Tai Kim, Tom Curran, Jung Je] The dendritic planarity of Purkinje cells is critical for cerebellar circuit formation. In the absence of Crk and CrkL, the Reelin pathway does not function resulting in partial Purkinje cell migration and defective dendritogenesis. However, the relationships among Purkinje cell migration, dendritic development and Reelin signaling have not been clearly delineated. Here, we use synchrotron X-ray microscopy to obtain 3-D images of Golgi-stained Purkinje cell dendrites. Purkinje cells that failed to migrate completely exhibited conical dendrites with abnormal 3-D arborization and reduced dendritic complexity. Furthermore, their spines were fewer in number with a distorted morphology. In contrast, Purkinje cells that migrated successfully displayed planar dendritic and spine morphologies similar to normal cells, despite reduced dendritic complexity. These results indicate that, during cerebellar formation, Purkinje cells migrate into an environment that supports development of dendritic planarity and spine formation. While Reelin signaling is important for the migration process, it does not make a direct major contribution to dendrite formation. The Author(s), 2014 Dendritic planarity nationallicence Purkinje cell nationallicence Reelin signaling nationallicence Migration nationallicence Synchrotron X-ray imaging nationallicence Kim Jinkyung X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut Park Tae-Ju Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia Research Institute, 19104, Philadelphia, PA, USA aut Kwon Namseop X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut Lee Dongmyeong APCTP & IES/NCSL, Department of Physics, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut Kim Seunghwan APCTP & IES/NCSL, Department of Physics, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut Kohmura Yoshiki RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, 679-5198, Sayo-gun, Hyogo, Japan aut Ishikawa Tetsuya RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, 679-5198, Sayo-gun, Hyogo, Japan aut Kim Kyong-Tai Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut Curran Tom Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia Research Institute, 19104, Philadelphia, PA, USA aut Je Jung X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2263-2273 1863-2653 220:4<2263 2015 220 429 https://doi.org/10.1007/s00429-014-0780-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0780-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Jinkyung X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut NATIONALLICENCE 700 1- Park Tae-Ju Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia Research Institute, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Kwon Namseop X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut NATIONALLICENCE 700 1- Lee Dongmyeong APCTP & IES/NCSL, Department of Physics, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut NATIONALLICENCE 700 1- Kim Seunghwan APCTP & IES/NCSL, Department of Physics, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut NATIONALLICENCE 700 1- Kohmura Yoshiki RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, 679-5198, Sayo-gun, Hyogo, Japan aut NATIONALLICENCE 700 1- Ishikawa Tetsuya RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, 679-5198, Sayo-gun, Hyogo, Japan aut NATIONALLICENCE 700 1- Kim Kyong-Tai Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut NATIONALLICENCE 700 1- Curran Tom Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia Research Institute, 19104, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Je Jung X-ray Imaging Center, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, South Korea aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2263-2273 1863-2653 220:4<2263 2015 220 429