caa a22 4500 60620265X CHVBK 20210128100954.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0781-1 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0781-1 Identification of CNS neurons with polysynaptic connections to both the sympathetic and parasympathetic innervation of the submandibular gland [Elektronische Daten] [N. Hettigoda, A. Fong, E. Badoer, M. McKinley, B. Oldfield, A. Allen] Coordinated modulation of sympathetic and parasympathetic nervous activity is required for physiological regulation of tissue function. Anatomically, whilst the peripheral sympathetic and parasympathetic pathways are separate, the distribution of premotor neurons in higher brain regions often overlaps. This co-distribution would enable coordinated regulation and might suggest individual premotor neurons could project to both sympathetic and parasympathetic outflows. To investigate this one submandibular gland was sympathectomized. One of two isogenic strains of the pseudorabies virus, expressing different fluorophores, was injected into the cut sympathetic nerve and the other into the submandibular gland. Independent labeling of the peripheral sympathetic and parasympathetic pathways was observed. Dual-labeled neurons were observed in many CNS regions known to be involved in regulating salivary function. We propose these observations highlight a common pattern of organization of the CNS, providing the anatomical framework for the fine control of organ function required for homeostatic regulation and the coordination of organ responses to enable complex behaviors. Springer-Verlag Berlin Heidelberg, 2014 Autonomic nervous system nationallicence Pseudorabies virus nationallicence Hypothalamic paraventricular nucleus nationallicence Raphe nuclei nationallicence Ventrolateral medulla nationallicence Salivary gland nationallicence Hettigoda N. Department of Physiology, University of Melbourne, 3010, Parkville, VIC, Australia aut Fong A. Department of Physiology, University of Melbourne, 3010, Parkville, VIC, Australia aut Badoer E. School of Medical Sciences, Health Innovations Research Institute, RMIT University, 3083, Bundoora, VIC, Australia aut McKinley M. Florey Neurosciences Institutes, University of Melbourne, 3010, Parkville, VIC, Australia aut Oldfield B. Department of Physiology, Monash University, 3800, Clayton, VIC, Australia aut Allen A. Department of Physiology, University of Melbourne, 3010, Parkville, VIC, Australia aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2103-2120 1863-2653 220:4<2103 2015 220 429 https://doi.org/10.1007/s00429-014-0781-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0781-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hettigoda N. Department of Physiology, University of Melbourne, 3010, Parkville, VIC, Australia aut NATIONALLICENCE 700 1- Fong A. Department of Physiology, University of Melbourne, 3010, Parkville, VIC, Australia aut NATIONALLICENCE 700 1- Badoer E. School of Medical Sciences, Health Innovations Research Institute, RMIT University, 3083, Bundoora, VIC, Australia aut NATIONALLICENCE 700 1- McKinley M. Florey Neurosciences Institutes, University of Melbourne, 3010, Parkville, VIC, Australia aut NATIONALLICENCE 700 1- Oldfield B. Department of Physiology, Monash University, 3800, Clayton, VIC, Australia aut NATIONALLICENCE 700 1- Allen A. Department of Physiology, University of Melbourne, 3010, Parkville, VIC, Australia aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2103-2120 1863-2653 220:4<2103 2015 220 429