caa a22 4500 606202765 CHVBK 20210128100954.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00429-014-0788-7 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0788-7 Galanin receptor 2-neuropeptide Y Y1 receptor interactions in the amygdala lead to increased anxiolytic actions [Elektronische Daten] [Manuel Narváez, Carmelo Millón, Dasiel Borroto-Escuela, Antonio Flores-Burgess, Luis Santín, Concepción Parrado, Belén Gago, Araceli Puigcerver, Kjell Fuxe, José Narváez, Zaida Díaz-Cabiale] Galanin (GAL) and neuropeptide Y (NPY) are neuropeptides involved in behaviors associated with anxiety. Both neuropeptides interact in several central functions. However, the potential behavioral and cellular interactions between them in anxiety are unknown. GAL was found to act through GAL receptor 2 (GALR2) to enhance NPYY1 receptor (NPYY1R)-mediated anxiolytic behaviors in rats. Using receptor autoradiography, c-Fos expression and in situ proximity ligation assay, the medial paracapsular intercalated nuclei of the amygdala were determined to be a key area in the interaction probably involving the formation of GALR2/NPYY1R heteroreceptor complexes. In cell cultures costimulation of GALR2 and NPYY1R induced changes in the functions of these receptors. The changes involved a potentiation of the decrease in the phosphorylation of CREB induced by NPYY1R and a delay in the internalization of NPYY1R. These results indicate that GALR2/NPYY1R interactions can provide a novel integrative amygdaloid mechanism in anxiety. Springer-Verlag Berlin Heidelberg, 2014 Galanin nationallicence Galanin receptor 2 nationallicence Neuropeptide Y nationallicence Neuropeptide Y Y1 receptor nationallicence Anxiety nationallicence Narváez Manuel Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut Millón Carmelo Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut Borroto-Escuela Dasiel Department of Neuroscience, Karolinska Institute, Stockholm, Sweden aut Flores-Burgess Antonio Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut Santín Luis Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Psicologia, Málaga, Spain aut Parrado Concepción Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut Gago Belén Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Ciencias, Málaga, Spain aut Puigcerver Araceli Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Psicologia, Málaga, Spain aut Fuxe Kjell Department of Neuroscience, Karolinska Institute, Stockholm, Sweden aut Narváez José Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut Díaz-Cabiale Zaida Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2289-2301 1863-2653 220:4<2289 2015 220 429 https://doi.org/10.1007/s00429-014-0788-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0788-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Narváez Manuel Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut NATIONALLICENCE 700 1- Millón Carmelo Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut NATIONALLICENCE 700 1- Borroto-Escuela Dasiel Department of Neuroscience, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 700 1- Flores-Burgess Antonio Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut NATIONALLICENCE 700 1- Santín Luis Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Psicologia, Málaga, Spain aut NATIONALLICENCE 700 1- Parrado Concepción Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut NATIONALLICENCE 700 1- Gago Belén Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Ciencias, Málaga, Spain aut NATIONALLICENCE 700 1- Puigcerver Araceli Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Psicologia, Málaga, Spain aut NATIONALLICENCE 700 1- Fuxe Kjell Department of Neuroscience, Karolinska Institute, Stockholm, Sweden aut NATIONALLICENCE 700 1- Narváez José Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut NATIONALLICENCE 700 1- Díaz-Cabiale Zaida Universidad de Málaga, Instituto de Investigación Biomédica, Facultad de Medicina, Málaga, Spain aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/4(2015-07-01), 2289-2301 1863-2653 220:4<2289 2015 220 429