caa a22 4500 606202803 CHVBK 20210128100954.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00429-014-0819-4 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0819-4 Altered neuronal architecture and plasticity in the visual cortex of adult MMP-3-deficient mice [Elektronische Daten] [Jeroen Aerts, Julie Nys, Lieve Moons, Tjing-Tjing Hu, Lutgarde Arckens] Matrix metalloproteinases (MMPs) are Zn2+-dependent endopeptidases considered to be essential for normal brain development and neuroplasticity by modulating extracellular matrix proteins, receptors, adhesion molecules, growth factors and cytoskeletal proteins. Specifically, MMP-3 has recently been implicated in synaptic plasticity, hippocampus-dependent learning and neuronal development and migration in the cerebellum. However, the function(s) of this enzyme in the neocortex is understudied. Therefore, we explored the phenotypical characteristics of the neuronal architecture and the capacity for experience-dependent cortical plasticity in the visual cortex of adult MMP-3-deficient (MMP-3−/−) mice. Golgi-Cox stainings revealed a significant reduction in apical dendritic length and an increased number of apical obliques for layer V pyramidal neurons in the visual cortex of adult MMP-3−/− mice compared to wild-type (WT) animals. In addition, a significant upregulation of both phosphorylated and non-phosphorylated neurofilament protein (NF)-high, phosphorylated NF-medium, NF-low and α-internexin was detected in the visual cortex of MMP-3−/− mice. To assess the effect of MMP-3 deficiency on cortical plasticity, we monocularly enucleated adult MMP-3−/− mice and analyzed the reactivation of the contralateral visual cortex 7weeks post-enucleation. In contrast to previous results in C57Bl/6J adult mice, activity remained confined to the binocular zone and did not expand into the monocular regions indicative for an aberrant open-eye potentiation. Permanent hypoactivity in the monocular cortex lateral and medial to V1 also indicated a lack of cross-modal plasticity. These observations demonstrate that genetic inactivation of MMP-3 has profound effects on the structural integrity and plasticity response of the visual cortex of adult mice. Springer-Verlag Berlin Heidelberg, 2014 Matrix metalloproteinase nationallicence Golgi-Cox nationallicence Apical dendrite nationallicence Neurofilament nationallicence Monocular enucleation nationallicence Multimodal nationallicence Aerts Jeroen Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut Nys Julie Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut Moons Lieve Laboratory of Neural Circuit Development and Regeneration, KU Leuven, Naamsestraat 61, 3000, Leuven, Belgium aut Hu Tjing-Tjing Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut Arckens Lutgarde Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2675-2689 1863-2653 220:5<2675 2015 220 429 https://doi.org/10.1007/s00429-014-0819-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0819-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aerts Jeroen Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Nys Julie Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Moons Lieve Laboratory of Neural Circuit Development and Regeneration, KU Leuven, Naamsestraat 61, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Hu Tjing-Tjing Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Arckens Lutgarde Laboratory of Neuroplasticity and Neuroproteomics, KU Leuven, Naamsestraat 59, 3000, Leuven, Belgium aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2675-2689 1863-2653 220:5<2675 2015 220 429