caa a22 4500 606202846 CHVBK 20210128100955.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00429-014-0837-2 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0837-2 A new automated 3D detection of synaptic contacts reveals the formation of cortico-striatal synapses upon cocaine treatment in vivo [Elektronische Daten] [Nicolas Heck, Marc Dos Santos, Brahim Amairi, Marine Salery, Antoine Besnard, Etienne Herzog, Thomas Boudier, Peter Vanhoutte, Jocelyne Caboche] Addiction can be considered as a form of neuronal adaptation within the reward circuitry. Upon psychostimulant administration, long-term behavioral adaptations are associated with synaptic plasticity and morphological changes of medium spiny neurons (MSN) from the striatum. Increased spine density onto MSN in response to chronic cocaine exposure in mice has been described for more than a decade, but no evidence indicates that these newly formed spines establish connections. We developed a method for labeling, automated detection and morphological analysis of synaptic contacts. Individual labeling of neurons in mice that express the Vesicular GLUtamate Transporter-1 fused to Venus allows visualization of both dendritic spines and axonal boutons. Automated three-dimensional segmentation and morphometric analysis retrieve information on thousands of synapses at high resolution. We used this method to demonstrate that new cortico-striatal connections are formed in the striatum upon chronic cocaine. We also show that the cortical input weight is preserved over other cerebral inputs and that the newly formed spines contact pre-existing axonal boutons. Our results pave the way for other studies, since our method can be applied to any other neuronal type as demonstrated herein for glutamatergic connections on pyramidal neurons and Purkinje cells. Springer-Verlag Berlin Heidelberg, 2014 Cocaine nationallicence Striatum nationallicence Dendritic spine nationallicence Axonal bouton nationallicence 3D segmentation nationallicence VGLUT1 nationallicence Heck Nicolas INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Dos Santos Marc INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Amairi Brahim INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Salery Marine INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Besnard Antoine INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Herzog Etienne Université Bordeaux, Institut Interdisciplinaire de Neurosciences, UMR 5297, 33000, Bordeaux, France aut Boudier Thomas Sorbonne Universités, UPMC Université Paris 06, UMR-S 8246, Neuroscience Paris Seine, 75005, Paris, France aut Vanhoutte Peter INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Caboche Jocelyne INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2953-2966 1863-2653 220:5<2953 2015 220 429 https://doi.org/10.1007/s00429-014-0837-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0837-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heck Nicolas INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Dos Santos Marc INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Amairi Brahim INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Salery Marine INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Besnard Antoine INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Herzog Etienne Université Bordeaux, Institut Interdisciplinaire de Neurosciences, UMR 5297, 33000, Bordeaux, France aut NATIONALLICENCE 700 1- Boudier Thomas Sorbonne Universités, UPMC Université Paris 06, UMR-S 8246, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Vanhoutte Peter INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 700 1- Caboche Jocelyne INSERM, UMR-S 1130, Neuroscience Paris Seine, 75005, Paris, France aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2953-2966 1863-2653 220:5<2953 2015 220 429