caa a22 4500 606202994 CHVBK 20210128100955.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00429-014-0816-7 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0816-7 Phosphorylation of S845 GluA1 AMPA receptors modulates spatial memory and structural plasticity in the ventral striatum [Elektronische Daten] [Valentina Ferretti, Valentina Perri, Alessia Cristofoli, Gisella Vetere, Paola Fragapane, Alberto Oliverio, Martine Teule, Andrea Mele] The function of AMPA receptors phosphorylation in synaptic plasticity has been dissected in many in vitro models but its role and dynamics on experience-dependent plasticity are still unclear. Here we studied the effects of AMPA receptor manipulations in the ventral striatum, where glutamatergic transmission is known to mediate spatial memory. We first demonstrate that intra-ventral striatal administrations of the AMPA receptors blocker, NBQX, dose dependently impair performance in the Morris water maze. We also report that spatial learning induced a time-limited increase in GluA1 phosphorylation in this same brain region. Finally, through focal, time-controlled ventral striatal administrations of an RNA aptamer interfering with GluA1-S845 phosphorylation, we demonstrate that phosphorylation at this site is a necessary requirement for spatial memory formation and for the synaptic remodeling underlying it. These results suggest that modulation of AMPA receptors by S845 phosphorylation could act as an essential starting signal leading to long-term stabilization of spatial memories. Springer-Verlag Berlin Heidelberg, 2014 NBQX nationallicence Nucleus accumbens nationallicence RNA aptamer nationallicence Dendritic spines nationallicence Ferretti Valentina Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut Perri Valentina Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut Cristofoli Alessia Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut Vetere Gisella Istituto Biologia Cellulare e Neurobiologia, CNR, Rome, Italy aut Fragapane Paola Istituto di Biologia e Patologia Molecolare, CNR, Rome, Italy aut Oliverio Alberto Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut Teule Martine Istituto Biologia Cellulare e Neurobiologia, CNR, Rome, Italy aut Mele Andrea Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2653-2661 1863-2653 220:5<2653 2015 220 429 https://doi.org/10.1007/s00429-014-0816-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0816-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ferretti Valentina Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut NATIONALLICENCE 700 1- Perri Valentina Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut NATIONALLICENCE 700 1- Cristofoli Alessia Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut NATIONALLICENCE 700 1- Vetere Gisella Istituto Biologia Cellulare e Neurobiologia, CNR, Rome, Italy aut NATIONALLICENCE 700 1- Fragapane Paola Istituto di Biologia e Patologia Molecolare, CNR, Rome, Italy aut NATIONALLICENCE 700 1- Oliverio Alberto Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut NATIONALLICENCE 700 1- Teule Martine Istituto Biologia Cellulare e Neurobiologia, CNR, Rome, Italy aut NATIONALLICENCE 700 1- Mele Andrea Dipartimento di Biologia e Biotecnologie, Centro di Ricerca in Neurobiologia "D. Bovet”, Università di Roma "La Sapienza”, 00185, Rome, Italy aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2653-2661 1863-2653 220:5<2653 2015 220 429