caa a22 4500 606203060 CHVBK 20210128100956.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00429-014-0823-8 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0823-8 Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism [Elektronische Daten] [Salvador Sierra, Natasha Luquin, Alberto Rico, Virginia Gómez-Bautista, Elvira Roda, Iria Dopeso-Reyes, Alfonso Vázquez, Eva Martínez-Pinilla, José Labandeira-García, Rafael Franco, José Lanciego] Although type 1 cannabinoid receptors (CB1Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors (CB2Rs) in neurons is still somewhat controversial. Taking advantage of newly designed CB1R and CB2R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of CB1Rs and CB2Rs, as well as CB1R-CB2R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of CB1Rs, CB2Rs and CB1R-CB2R heteromers was found in dyskinetic animals, mimicking the observed reduction in CB1R and CB2R mRNA expression levels. The fact that chronic levodopa treatment disrupted CB1R-CB2R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers. The Author(s), 2014 GPCRs nationallicence Cannabis nationallicence Cannabinoid receptor heteromer nationallicence Dyskinesia nationallicence Globus pallidus nationallicence MPTP nationallicence Sierra Salvador Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Luquin Natasha Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Rico Alberto Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Gómez-Bautista Virginia Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Roda Elvira Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Dopeso-Reyes Iria Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Vázquez Alfonso Department of Neurosurgery, Complejo Hospitalario de Navarra, Pamplona, Spain aut Martínez-Pinilla Eva Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Labandeira-García José Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED), Madrid, Spain aut Franco Rafael Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Lanciego José Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2721-2738 1863-2653 220:5<2721 2015 220 429 https://doi.org/10.1007/s00429-014-0823-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0823-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sierra Salvador Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Luquin Natasha Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Rico Alberto Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Gómez-Bautista Virginia Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Roda Elvira Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Dopeso-Reyes Iria Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Vázquez Alfonso Department of Neurosurgery, Complejo Hospitalario de Navarra, Pamplona, Spain aut NATIONALLICENCE 700 1- Martínez-Pinilla Eva Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Labandeira-García José Network Center for Biomedical Research in Neurodegenerative Diseases (CIBERNED), Madrid, Spain aut NATIONALLICENCE 700 1- Franco Rafael Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 700 1- Lanciego José Neurosciences Division, Center for Applied Medical Research (CIMA), University of Navarra, Pio XII Avenue 55, 31008, Pamplona, Spain aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2721-2738 1863-2653 220:5<2721 2015 220 429