caa a22 4500 606203184 CHVBK 20210128100956.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00429-014-0824-7 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0824-7 Localisation and stress-induced plasticity of GABAA receptor subunits within the cellular networks of the mouse dorsal raphe nucleus [Elektronische Daten] [Nicole Corteen, Jessica Carter, Uwe Rudolph, Delia Belelli, Jeremy Lambert, Jerome Swinny] The dorsal raphe nucleus (DRN) provides the major source of serotonin to the central nervous system (CNS) and modulates diverse neural functions including mood. Furthermore, DRN cellular networks are engaged in the stress-response at the CNS level allowing for adaptive behavioural responses, whilst stress-induced dysregulation of DRN and serotonin release is implicated in psychiatric disorders. Therefore, identifying the molecules regulating DRN activity is fundamental to understand DRN function in health and disease. GABAA receptors (GABAARs) allow for brain region, cell type and subcellular domain-specific GABA-mediated inhibitory currents and are thus key regulators of neuronal activity. Yet, the GABAAR subtypes expressed within the neurochemically diverse cell types of the mouse DRN are poorly described. In this study, immunohistochemistry and confocal microscopy revealed that all serotonergic neurons expressed immunoreactivity for the GABAAR alpha2 and 3 subunits, although the respective signals were co-localised to varying degrees with inhibitory synaptic marker proteins. Only a topographically located sub-population of serotonergic neurons exhibited GABAAR alpha1 subunit immunoreactivity. However, all GABAergic as well as non-GABAergic, non-serotonergic neurons within the DRN expressed GABAAR alpha1 subunit immunoreactivity. Intriguingly, immunoreactivity for the GABAAR gamma2 subunit was enriched on GABAergic rather than serotonergic neurons. Finally, repeated restraint stress increased the expression of the GABAAR alpha3 subunit at the mRNA and protein level. The study demonstrates the identity and location of distinct GABAAR subunits within the cellular networks of the mouse DRN and that stress impacts on the expression levels of particular subunits at the gene and protein level. Springer-Verlag Berlin Heidelberg, 2014 Serotonin nationallicence Immunohistochemistry nationallicence Anxiety nationallicence Benzodiazepine nationallicence Depression nationallicence Corteen Nicole Institute for Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, Portsmouth, UK aut Carter Jessica Institute for Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, Portsmouth, UK aut Rudolph Uwe Laboratory of Genetic Neuropharmacology, McLean Hospital and Department of Psychiatry, Harvard Medical School, 02478, Belmont, MA, USA aut Belelli Delia Division of Neuroscience, Medical Research Institute, Ninewells Hospital and Medical School, Ninewells Hospital, Dundee University, DD19SY, Dundee, UK aut Lambert Jeremy Division of Neuroscience, Medical Research Institute, Ninewells Hospital and Medical School, Ninewells Hospital, Dundee University, DD19SY, Dundee, UK aut Swinny Jerome Institute for Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, Portsmouth, UK aut Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2739-2763 1863-2653 220:5<2739 2015 220 429 https://doi.org/10.1007/s00429-014-0824-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0824-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Corteen Nicole Institute for Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, Portsmouth, UK aut NATIONALLICENCE 700 1- Carter Jessica Institute for Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, Portsmouth, UK aut NATIONALLICENCE 700 1- Rudolph Uwe Laboratory of Genetic Neuropharmacology, McLean Hospital and Department of Psychiatry, Harvard Medical School, 02478, Belmont, MA, USA aut NATIONALLICENCE 700 1- Belelli Delia Division of Neuroscience, Medical Research Institute, Ninewells Hospital and Medical School, Ninewells Hospital, Dundee University, DD19SY, Dundee, UK aut NATIONALLICENCE 700 1- Lambert Jeremy Division of Neuroscience, Medical Research Institute, Ninewells Hospital and Medical School, Ninewells Hospital, Dundee University, DD19SY, Dundee, UK aut NATIONALLICENCE 700 1- Swinny Jerome Institute for Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, St Michael's Building, White Swan Road, PO1 2DT, Portsmouth, UK aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/5(2015-09-01), 2739-2763 1863-2653 220:5<2739 2015 220 429