caa a22 4500 606203265 CHVBK 20210128100956.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00429-013-0690-8 doi (NATIONALLICENCE)springer-10.1007/s00429-013-0690-8 A quantitative analysis of cellular and synaptic localization of GAT-1 and GAT-3 in rat neocortex [Elektronische Daten] [Marcello Melone, Silvia Ciappelloni, Fiorenzo Conti] High-affinity plasma membrane GABA transporters GAT-1 and GAT-3 contribute to the modulation of GABA-mediated inhibition in adult mammalian cerebral cortex. How GATs regulate inhibition in neocortical circuits remains however poorly understood for the lack of information on key localizational features. In this study, we used quantitative pre- and post-embedding electron microscopy to define the distribution of GAT-1 and GAT-3 in elements contributing to synapses and to unveil their ultrastructural organization at adult cortical GABAergic synapses. GAT-1 and GAT-3 were found in both neuronal and astrocytic processes: GAT-1 was prevalently segregated in neuronal elements and in profiles contributing to synapses, whereas GAT-3 was mostly expressed in astrocytes and did not exhibit a preferential distribution in elements contributing to synapses. Analysis of the ultrastructural distribution of GAT-1 and GAT-3 in the plasma membrane of axon terminals and perisynaptic astrocytic processes of symmetric synapses in relation to the active zone revealed that GAT-1 was more concentrated in restricted perisynaptic and extrasynaptic regions, whereas GAT-3 was prominent in extrasynaptic areas. These studies provide a basis for understanding the role GAT-1 and GAT-3 play in the modulation of GABA-mediated phasic and tonic inhibition in cerebral cortex. Springer-Verlag Berlin Heidelberg, 2013 GABA nationallicence GABA transporters nationallicence Cerebral cortex nationallicence Synapses nationallicence Tonic inhibition nationallicence Phasic inhibition nationallicence Melone Marcello Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Università Politecnica delle Marche, 60026, Ancona, Italy aut Ciappelloni Silvia Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Università Politecnica delle Marche, 60026, Ancona, Italy aut Conti Fiorenzo Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Università Politecnica delle Marche, 60026, Ancona, Italy aut Brain Structure and Function Springer Berlin Heidelberg 220/2(2015-03-01), 885-897 1863-2653 220:2<885 2015 220 429 https://doi.org/10.1007/s00429-013-0690-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-013-0690-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Melone Marcello Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Università Politecnica delle Marche, 60026, Ancona, Italy aut NATIONALLICENCE 700 1- Ciappelloni Silvia Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Università Politecnica delle Marche, 60026, Ancona, Italy aut NATIONALLICENCE 700 1- Conti Fiorenzo Department of Experimental and Clinical Medicine, Section of Neuroscience and Cell Biology, Università Politecnica delle Marche, 60026, Ancona, Italy aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/2(2015-03-01), 885-897 1863-2653 220:2<885 2015 220 429