caa a22 4500 606203451 CHVBK 20210128100957.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00429-014-0711-2 doi (NATIONALLICENCE)springer-10.1007/s00429-014-0711-2 The transient receptor potential vanilloid-1 is localized at excitatory synapses in the mouse dentate gyrus [Elektronische Daten] [Nagore Puente, Leire Reguero, Izaskun Elezgarai, Miren-Josune Canduela, Juan Mendizabal-Zubiaga, Almudena Ramos-Uriarte, Emilio Fernández-Espejo, Pedro Grandes] The transient receptor potential vanilloid type 1 (TRPV1) is a non-selective cation channel that plays an important role in pain perception and modulates neurotransmitter release and synaptic plasticity in the brain. TRPV1 function must lay on its anatomical distribution in the peripheral and central nervous system regions involved in the physiological roles of the channel. However, the anatomical localization of TRPV1 is well established in the periphery, but in the brain it is a matter of debate. While some studies support the presence of TRPV1 in several brain regions, recent evidences suggest a restricted distribution of the channel in the central nervous system. To investigate to what extent central TRPV1 function stands on a precise brain distribution of the channel, we examined the mouse hippocampal dentate molecular layer (ML) where TRPV1 mediates long-term synaptic plasticity. Using pre-embedding immunocytochemistry for high resolution electron microscopy, we show that TRPV1 immunoparticles are highly concentrated in postsynaptic dendritic spines to asymmetric perforant path synapses in the outer 2/3 of the ML. However, TRPV1 is poorly expressed at the excitatory hilar mossy cell synapses in the inner 1/3 of this layer. Importantly, the TRPV1 pattern distribution disappeared in the ML of TRPV1-knockout mice. Taken together, these findings support the notion of the presence of TRPV1 in a brain region where the channel has been shown to have a functional role, such as the perforant path synapses in the hippocampal dentate ML. Springer-Verlag Berlin Heidelberg, 2014 Hippocampus nationallicence Perforant path nationallicence Ion channel nationallicence Immunocytochemistry nationallicence Electron microscopy nationallicence 2-AG : 2-Arachidonoylglycerol nationallicence ABC : Avidin-biotin peroxidase complex nationallicence BSA : Bovine serum albumin nationallicence DGL-α : Diacylglycerol lipase-α nationallicence LTD : Long-term depression nationallicence mGluR : Metabotropic glutamate receptor nationallicence ML : Molecular layer nationallicence NAPE-PLD : N-Acyl phosphatidylethanolamine phospholipase D nationallicence RT : Room temperature nationallicence PB : Phosphate buffer nationallicence PBS : Phosphate buffered saline nationallicence TBS : Tris-HCl buffered saline nationallicence TRPV1 : Transient receptor potential vanilloid type 1 nationallicence Puente Nagore Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Reguero Leire Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Elezgarai Izaskun Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Canduela Miren-Josune Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Mendizabal-Zubiaga Juan Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Ramos-Uriarte Almudena Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Fernández-Espejo Emilio Department of Medical Physiology and Biophysics, Faculty of Medicine, Universidad de Sevilla, Seville, Spain aut Grandes Pedro Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut Brain Structure and Function Springer Berlin Heidelberg 220/2(2015-03-01), 1187-1194 1863-2653 220:2<1187 2015 220 429 https://doi.org/10.1007/s00429-014-0711-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-014-0711-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Puente Nagore Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 700 1- Reguero Leire Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 700 1- Elezgarai Izaskun Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 700 1- Canduela Miren-Josune Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 700 1- Mendizabal-Zubiaga Juan Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 700 1- Ramos-Uriarte Almudena Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 700 1- Fernández-Espejo Emilio Department of Medical Physiology and Biophysics, Faculty of Medicine, Universidad de Sevilla, Seville, Spain aut NATIONALLICENCE 700 1- Grandes Pedro Department of Neurosciences, Faculty of Medicine and Dentistry, University of the Basque Country UPV/EHU, 48940, Leioa, Spain aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/2(2015-03-01), 1187-1194 1863-2653 220:2<1187 2015 220 429