caa a22 4500 606203591 CHVBK 20210128100958.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00429-013-0689-1 doi (NATIONALLICENCE)springer-10.1007/s00429-013-0689-1 Selective presence of a giant saccular organelle in the axon initial segment of a subpopulation of layer V pyramidal neurons [Elektronische Daten] [Alejandro Antón-Fernández, Pablo Rubio-Garrido, Javier DeFelipe, Alberto Muñoz] Recently it has been shown that a giant saccular organelle (GSO) of unknown function is present in the axon initial segment (AIS) of an uncharacterized population of pyramidal cells of the rodent neocortex. Using tract-tracing methods and immunocytochemistry, in the present study we show that in rodents this GSO is present in the AIS of subpopulations of layer V pyramidal neurons projecting to various subcortical, non-thalamic targets, including the spinal cord. GSO-containing neurons express SMI32 and some of them are under the control of the Thy-1 gene promoter. In addition, our results demonstrate that the GSO expresses the inositol 1,4,5-triphosphate receptor 1 (IP3R1) and the sarco (endo) plasmic reticulum Ca2+ ATPase 2, both in rodent and human neocortex. These results indicate the involvement of the GSO in the regulation of Ca2+ levels in the AIS in a particular subpopulation of layer V neurons that give rise to subcortical non-thalamic descending projections. Springer-Verlag Berlin Heidelberg, 2013 Calcium nationallicence Cerebral cortex nationallicence Corticospinal tract nationallicence SERCA nationallicence Synaptopodin nationallicence IP3R1 nationallicence Antón-Fernández Alejandro Instituto Cajal, CSIC, 28002, Madrid, Spain aut Rubio-Garrido Pablo Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma, Madrid, Spain aut DeFelipe Javier Instituto Cajal, CSIC, 28002, Madrid, Spain aut Muñoz Alberto Instituto Cajal, CSIC, 28002, Madrid, Spain aut Brain Structure and Function Springer Berlin Heidelberg 220/2(2015-03-01), 869-884 1863-2653 220:2<869 2015 220 429 https://doi.org/10.1007/s00429-013-0689-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00429-013-0689-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Antón-Fernández Alejandro Instituto Cajal, CSIC, 28002, Madrid, Spain aut NATIONALLICENCE 700 1- Rubio-Garrido Pablo Departamento de Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma, Madrid, Spain aut NATIONALLICENCE 700 1- DeFelipe Javier Instituto Cajal, CSIC, 28002, Madrid, Spain aut NATIONALLICENCE 700 1- Muñoz Alberto Instituto Cajal, CSIC, 28002, Madrid, Spain aut NATIONALLICENCE 773 0- Brain Structure and Function Springer Berlin Heidelberg 220/2(2015-03-01), 869-884 1863-2653 220:2<869 2015 220 429