caa a22 4500 606204709 CHVBK 20210128101004.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10388-014-0459-z doi (NATIONALLICENCE)springer-10.1007/s10388-014-0459-z Histopathological and immunohistochemical characteristics of superficial squamous cell carcinoma forming droplet infiltration: indicator of lymph node metastasis of the esophagus [Elektronische Daten] [Ryuta Nakao, Yasuko Fujita, Nobuaki Yagi, Eiichi Konishi, Akio Yanagisawa] Background: Droplet infiltration (DI) is a tumor infiltration pattern observed in superficial squamous cell carcinoma (SSCC) of the esophagus. Lymph node metastasis (LNM) is related to three reported features of DI (DILFs): longitudinal diameter ≤20μm (DIsLF); number of constituent cells ≤4 (DInLF); distance from the primary focus ≥200μm (DIdLF). The aim of this study was to clarify the characteristics of SSCC with high risk of LNM, by analyzing DILF-forming SSCC histopathologically and immunohistochemically. Methods: Forty-five SSCC invading the muscularis mucosa or submucosa were divided into two groups with and without above-mentioned three DILFs. The Ki-67 labeling index (LI) and the expression of cytokeratin (CK) 13, 14, 17, and 19 were compared in the paired. Results: The Ki-67 LI was elevated in SSCC with DIsLF and with DInLF (P<0.001, each), but no significant difference was observed between SSCC with and without DIdLF. The expression of CK13 was decreased in SSCC with DIsLF (P<0.01) and with DInLF (P<0.001), but no significant difference was observed between SSCC with and without DIdLF. For CK14, CK17, and CK19, no significant difference was observed between SSCC with and without any DILF. Conclusions: The results indicated that DIsLF and DInLF are not only LNM predictors, but useful morphological markers reflecting excessive cell proliferation, and that the reduced expression of CK13 is closely associated with DIsLF and DInLF formation at the invasive front. Therefore, the elevation of Ki-67 LI and reduced expression of CK13 suggest higher risk of LNM in esophageal SSCC. The Japan Esophageal Society and Springer Japan, 2014 Esophageal superficial squamous cell carcinoma nationallicence Endoscopic submucosal dissection nationallicence Droplet infiltration nationallicence Ki-67 nationallicence CK13 nationallicence Nakao Ryuta Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut Fujita Yasuko Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut Yagi Nobuaki Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, Japan aut Konishi Eiichi Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut Yanagisawa Akio Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut Esophagus Springer Japan 12/2(2015-04-01), 175-183 1612-9059 12:2<175 2015 12 10388 https://doi.org/10.1007/s10388-014-0459-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10388-014-0459-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakao Ryuta Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut NATIONALLICENCE 700 1- Fujita Yasuko Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut NATIONALLICENCE 700 1- Yagi Nobuaki Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, Japan aut NATIONALLICENCE 700 1- Konishi Eiichi Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut NATIONALLICENCE 700 1- Yanagisawa Akio Department of Surgical Pathology, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, 602-8566, Kyoto, Japan aut NATIONALLICENCE 773 0- Esophagus Springer Japan 12/2(2015-04-01), 175-183 1612-9059 12:2<175 2015 12 10388