caa a22 4500 606207481 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2277-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2277-5 HRA2pl peptide: a fusion inhibitor for human metapneumovirus produced in tobacco plants by transient transformation [Elektronische Daten] [Verónica Márquez-Escobar, Rocío Tirado-Mendoza, Daniel Noyola, Abel Gutiérrez-Ortega, Ángel Alpuche-Solís] Main conclusion : The HRA2pl peptide expressed by transient transformation in N. tabacum plants is capable of inhibiting the binding of the human metapneumovirus to HEp-2 cells at the fusion stage. Human metapneumovirus (hMPV) is an agent responsible for acute respiratory infections that mainly affects children under 3years, the elderly and immunocompromised patients. In children younger than 5years, respiratory tract infections account for 20% of deaths worldwide. However, there is currently no treatment or vaccine available against hMPV. The production of a safe, efficient and low cost treatment against this virus is a current challenge. Plants provide a system for recombinant protein production that is cost effective and is easier to scale up to an industrial level than other platforms; in addition, the plant tissue may be used as raw food, dried or, alternatively, proteins may be partially or fully purified and administered in aerosol or capsules as dry powder. In this study, we designed a gene expressing an antiviral peptide against hMPV based on the heptad repeat A domain of the F protein of the virus. We produced the recombinant peptide by a viral transient expression system (Magnifection®) in Nicotiana tabacum plants. The efficacy of this antiviral peptide was confirmed by in vitro assays in HEp-2 cell line. This is a promising result that can offer a prophylactic approach against hMPV. Springer-Verlag Berlin Heidelberg, 2015 Transient transformation nationallicence Antiviral peptide nationallicence hMPV nationallicence HRA domain nationallicence Márquez-Escobar Verónica División de Biología Molecular, IPICYT/Instituto Potosino de Investigación Científica y Tecnológica A. C., Camino a la Presa de San José 2055, 78216, San Luis Potosí, San Luis Potosí, Mexico aut Tirado-Mendoza Rocío Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Col. Universidad Nacional Autónoma de México, 04510, Delegación Coyoacán, Distrito Federal, Mexico aut Noyola Daniel Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210, San Luis Potosí, San Luis Potosí, Mexico aut Gutiérrez-Ortega Abel Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico aut Alpuche-Solís Ángel División de Biología Molecular, IPICYT/Instituto Potosino de Investigación Científica y Tecnológica A. C., Camino a la Presa de San José 2055, 78216, San Luis Potosí, San Luis Potosí, Mexico aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 69-76 0032-0935 242:1<69 2015 242 425 https://doi.org/10.1007/s00425-015-2277-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2277-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Márquez-Escobar Verónica División de Biología Molecular, IPICYT/Instituto Potosino de Investigación Científica y Tecnológica A. C., Camino a la Presa de San José 2055, 78216, San Luis Potosí, San Luis Potosí, Mexico aut NATIONALLICENCE 700 1- Tirado-Mendoza Rocío Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Col. Universidad Nacional Autónoma de México, 04510, Delegación Coyoacán, Distrito Federal, Mexico aut NATIONALLICENCE 700 1- Noyola Daniel Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza 2405, 78210, San Luis Potosí, San Luis Potosí, Mexico aut NATIONALLICENCE 700 1- Gutiérrez-Ortega Abel Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Col. Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico aut NATIONALLICENCE 700 1- Alpuche-Solís Ángel División de Biología Molecular, IPICYT/Instituto Potosino de Investigación Científica y Tecnológica A. C., Camino a la Presa de San José 2055, 78216, San Luis Potosí, San Luis Potosí, Mexico aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 69-76 0032-0935 242:1<69 2015 242 425