caa a22 4500 60620749X CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2289-1 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2289-1 Genetic reduction of inositol triphosphate (InsP3) increases tolerance of tomato plants to oxidative stress [Elektronische Daten] [Mohammad Alimohammadi, Mohamed Lahiani, Mariya Khodakovskaya] Main conclusion : We demonstrate here that the reduction of InsP 3 , the key component of the phosphoinositol pathway, results in changes in ROS-scavenging machinery and, subsequently, increases the tolerance of tomato plants to light stress. Different plant stress signaling pathways share similar elements and, therefore, ‘cross-talk' between the various pathways can exist. Links between the phosphoinositol signaling pathway and light signaling were recently found. Tomato plants expressing InsP 5-ptase and exhibiting reduction in the level of inositol 1,4,5-triphosphate (InsP3) demonstrated enhanced tolerance to stress caused by continuous light exposure. To understand the molecular basis of observed stress tolerance in tomato lines with decreased amount of InsP3, we monitored the expression of enzymatic antioxidants as well as important factors in light signaling associated with non-enzymatic antioxidants (secondary metabolites). Here, we demonstrated that InsP 5-ptase transgenic plants accumulate less hydroxide peroxide and maintain higher chlorophyll content during stress caused by continuous light exposure. This observation can be explained by documented activation of multiple enzymatic antioxidants (LeAPX1, SICAT2, LeSOD) at levels of gene expression and enzymatic activities during continuous light exposure. In addition, we noticed the up-regulation of photoreceptors LePHYB and LeCHS1, key enzymes in flavonoid biosynthesis pathway, transcription factors LeHY5, SIMYB12, and early light-inducible protein (LeELIP) genes in transgenic tomato seedlings exposed to blue or red light. Our study confirmed the existence of a correlation between phosphoinositol signaling pathway modification, increased tolerance to stress caused by continuous light exposure, activation of ROS-scavenging enzymes, and up-regulation of molecular activators of non-enzymatic antioxidants in InsP 5-ptase expressing tomato lines. Springer-Verlag Berlin Heidelberg, 2015 Oxidative stress nationallicence Phosphoinositol pathway nationallicence Light signaling nationallicence Secondary metabolism nationallicence ROS-scavenging enzymes nationallicence InsP3 : Inositol 1,4,5-triphosphate nationallicence InsP 5-ptase : Inositol trisphosphate 5 phosphatase nationallicence SOD : Superoxide dismutase nationallicence CAT : Catalase nationallicence APX : Ascorbate peroxidase nationallicence LeCHS1 : Lycopersicon esculentum chalcone synthase nationallicence LeHY5 : Lycopersicon esculentum long hypocotyl 5 transcription factor nationallicence LePHYB : Lycopersicon esculentum photochrome B nationallicence SICRY1 : Solanum lycopersicum cryptochrome 1 nationallicence LeELIP : Lycopersicon esculentum early light-inducible protein nationallicence Alimohammadi Mohammad Department of Biology, University of Arkansas at Little Rock, Little Rock, USA aut Lahiani Mohamed Department of Biology, University of Arkansas at Little Rock, Little Rock, USA aut Khodakovskaya Mariya Department of Biology, University of Arkansas at Little Rock, Little Rock, USA aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 123-135 0032-0935 242:1<123 2015 242 425 https://doi.org/10.1007/s00425-015-2289-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2289-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alimohammadi Mohammad Department of Biology, University of Arkansas at Little Rock, Little Rock, USA aut NATIONALLICENCE 700 1- Lahiani Mohamed Department of Biology, University of Arkansas at Little Rock, Little Rock, USA aut NATIONALLICENCE 700 1- Khodakovskaya Mariya Department of Biology, University of Arkansas at Little Rock, Little Rock, USA aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 123-135 0032-0935 242:1<123 2015 242 425