caa a22 4500 606207503 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2314-4 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2314-4 Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage [Elektronische Daten] [Dipak Gayen, Nusrat Ali, Sailendra Sarkar, Swapan Datta, Karabi Datta] Main conclusion : Down-regulation of lipoxygenase enzyme activity reduces degradation of carotenoids of bio-fortified rice seeds which would be an effective tool to reduce huge post-harvest and economic losses of bio-fortified rice seeds during storage. Bio-fortified provitamin A-enriched rice line (golden rice) expressing higher amounts of β-carotene in the rice endosperm provides vitamin A for human health. However, it is already reported that degradation of carotenoids during storage is a major problem. The gene responsible for degradation of carotenoids during storage has remained largely unexplored till now. In our previous study, it has been shown that r9-LOX1 gene is responsible for rice seed quality deterioration. In the present study, we attempted to investigate if r9-LOX1 gene has any role in degradation of carotenoids in rice seeds during storage. To establish our hypothesis, the endogenous lipoxygenase (LOX) activity of high-carotenoid golden indica rice seed was silenced by RNAi technology using aleurone layer and embryo-specific Oleosin-18 promoter. To check the storage stability, LOX enzyme down-regulated high-carotenoid T3 transgenic rice seeds were subjected to artificial aging treatment. The results obtained from biochemical assays (MDA, ROS) also indicated that after artificial aging, the deterioration of LOX-RNAi lines was considerably lower compared to β-carotene-enriched transgenic rice which had higher LOX activity in comparison to LOX-RNAi lines. Furthermore, it was also observed by HPLC analysis that down-regulation of LOX gene activity decreases co-oxidation of β-carotene in LOX-RNAi golden rice seeds as compared to the β-carotene-enriched transgenic rice, after artificial aging treatment. Therefore, our study substantially establishes and verifies that LOX is a key enzyme for catalyzing co-oxidation of β-carotene and has a significant role in deterioration of β-carotene levels in the carotenoid-enriched golden rice. Springer-Verlag Berlin Heidelberg, 2015 RNAi nationallicence β-carotene nationallicence Provitamin A nationallicence Lipoxygenase nationallicence Post-harvest deterioration nationallicence Artificial aging nationallicence LOX : Lipoxygenase nationallicence MDA : Malondialdehyde nationallicence ROS : Reactive oxygen species nationallicence VAD : Vit-A deficiency nationallicence DCFDA : 2′, 7′-Dichlorofluorescin diacetate nationallicence Gayen Dipak Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut Ali Nusrat Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut Sarkar Sailendra Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut Datta Swapan Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut Datta Karabi Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 353-363 0032-0935 242:1<353 2015 242 425 https://doi.org/10.1007/s00425-015-2314-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2314-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gayen Dipak Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Ali Nusrat Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Sarkar Sailendra Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Datta Swapan Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut NATIONALLICENCE 700 1- Datta Karabi Laboratory of Translational Research on Transgenic Crops, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, West Bengal, India aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 353-363 0032-0935 242:1<353 2015 242 425