caa a22 4500 606207511 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2285-5 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2285-5 A fungal transcription factor gene is expressed in plants from its own promoter and improves drought tolerance [Elektronische Daten] [Félix Martínez, Anjuman Arif, Sergio Nebauer, Eduardo Bueso, Rashid Ali, Consuelo Montesinos, Veronique Brunaud, Jesús Muñoz-Bertomeu, Ramón Serrano] Main conclusion : A fungal gene encoding a transcription factor is expressed from its own promoter in Arabidopsis phloem and improves drought tolerance by reducing transpiration and increasing osmotic potential. Horizontal gene transfer from unrelated organisms has occurred in the course of plant evolution, suggesting that some foreign genes may be useful to plants. The CtHSR1 gene, previously isolated from the halophytic yeast Candida tropicalis, encodes a heat-shock transcription factor-related protein. CtHSR1, with expression driven by its own promoter or by the Arabidopsis UBQ10 promoter, was introduced into the model plant Arabidopsis thaliana by Agrobacterium tumefaciens-mediated transformation and the resulting transgenic plants were more tolerant to drought than controls. Fusions of the CtHSR1 promoter with β-glucuronidase reporter gene indicated that this fungal promoter drives expression to phloem tissues. A chimera of CtHSR1 and green fluorescence protein is localized at the cell nucleus. The physiological mechanism of drought tolerance in transgenic plants is based on reduced transpiration (which correlates with decreased opening of stomata and increased levels of jasmonic acid) and increased osmotic potential (which correlates with increased proline accumulation). Transcriptomic analysis indicates that the CtHSR1 transgenic plants overexpressed a hundred of genes, including many relevant to stress defense such as LOX4 (involved in jasmonic acid synthesis) and P5CS1 (involved in proline biosynthesis). The promoters of the induced genes were enriched in upstream activating sequences for water stress induction. These results demonstrate that genes from unrelated organisms can have functional expression in plants from its own promoter and expand the possibilities of useful transgenes for plant biotechnology. Springer-Verlag Berlin Heidelberg, 2015 Arabidopsis nationallicence Candida tropicalis nationallicence Horizontal gene transfer nationallicence Jasmonic acid nationallicence Proline nationallicence Water relations nationallicence ABA : Abscisic acid nationallicence DAPI : 4′,6-diamino-2-phenylindole nationallicence GFP : Green fluorescent protein nationallicence GUS : β-Glucuronidase nationallicence HSE : Heat-shock element nationallicence HSF : Heat-shock transcription factor nationallicence IAA : Indolacetic acid nationallicence JA : Jasmonic acid nationallicence Martínez Félix Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut Arif Anjuman National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan aut Nebauer Sergio Departamento de Producción Vegetal, Universidad Politécnica de Valencia, ETSIAMN, Camino de Vera, 46022, Valencia, Spain aut Bueso Eduardo Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut Ali Rashid Department of Allied Health Sciences, University of Connecticut, 06269, Storrs, CT, USA aut Montesinos Consuelo Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut Brunaud Veronique Unité de Recherche en Génomique Végétale Plant Genomics, 91057, Evry, France aut Muñoz-Bertomeu Jesús Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut Serrano Ramón Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 39-52 0032-0935 242:1<39 2015 242 425 https://doi.org/10.1007/s00425-015-2285-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2285-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez Félix Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Arif Anjuman National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan aut NATIONALLICENCE 700 1- Nebauer Sergio Departamento de Producción Vegetal, Universidad Politécnica de Valencia, ETSIAMN, Camino de Vera, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Bueso Eduardo Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Ali Rashid Department of Allied Health Sciences, University of Connecticut, 06269, Storrs, CT, USA aut NATIONALLICENCE 700 1- Montesinos Consuelo Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Brunaud Veronique Unité de Recherche en Génomique Végétale Plant Genomics, 91057, Evry, France aut NATIONALLICENCE 700 1- Muñoz-Bertomeu Jesús Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut NATIONALLICENCE 700 1- Serrano Ramón Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Camino de Vera, 46022, Valencia, Spain aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 39-52 0032-0935 242:1<39 2015 242 425