caa a22 4500 60620752X CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2295-3 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2295-3 Biochemical characterization of caffeoyl coenzyme A 3- O -methyltransferase from wheat [Elektronische Daten] [Qing-Hu Ma, Hao-Ran Luo] Main conclusion : TaCCoAOMT1 is located in wheat chromosome 7A and highly expressed in stem and root. It is important for lignin biosynthesis, and associated with stem maturity but not lodging resistance. Caffeoyl coenzyme A 3-O-methyltransferases (CCoAOMTs) are one important class of enzymes to carry out the transfer of the methyl group from S-adenosylmethionine to the hydroxyl group, and play important roles in lignin and flavonoids biosynthesis. In the present study, sequences for CCoAOMT from the wheat genome were analyzed. One wheat CCoAOMT that belonged to bona fide subclade involved in lignin biosynthesis, namely TaCCoAOMT1, was obtained by the prokaryotic expression in E. coli. The three-dimensional structure prediction showed a highly similar structure of TaCCoAOMT1 with MsCCoAOMT. Recombinant TaCCoAOMT1 protein could only use caffeoyl CoA and 5-hydroxyferuloyl CoA as effective substrates and caffeoyl CoA as the best substrate. TaCCoAOMT1 had a narrow optimal pH and thermal stability. The TaCCoAOMT1 gene was highly expressed in wheat stem and root tissues, paralleled CCoAOMT enzyme activity. TaCCoAOMT1 mRNA abundance and enzyme activity increased linearly with stem maturity, but showed little difference between wheat lodging-resistant (H4546) and lodging-sensitive (C6001) cultivars in elongation, heading and milky stages. These data suggest that TaCCoAOMT1 is an important CCoAOMT for lignin biosynthesis that is critical for stem development, but not directly associated with lodging-resistant trait in wheat. Springer-Verlag Berlin Heidelberg, 2015 Caffeoyl coenzyme A 3- O -methyltransferase (CCoAOMT) nationallicence Lignin biosynthesis nationallicence Lodging resistance nationallicence Stem development nationallicence Triticum nationallicence CCoAOMT : Caffeoyl coenzyme A 3-O-methyltransferase nationallicence COMT : Caffeic acid 3-O-methyltransferase nationallicence EST : Expressed sequence tag nationallicence NCBI : National Center for Biotechnology Information nationallicence OMT : O-Methyltransferase nationallicence ORF : Open reading frame nationallicence PCR : Polymerase chain reaction nationallicence SAM : S-Adenosylmethionine nationallicence TaGI : Triticum aestivum gene index database nationallicence Ma Qing-Hu Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093, Beijing, China aut Luo Hao-Ran Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093, Beijing, China aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 113-122 0032-0935 242:1<113 2015 242 425 https://doi.org/10.1007/s00425-015-2295-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2295-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Qing-Hu Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 700 1- Luo Hao-Ran Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, 100093, Beijing, China aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 113-122 0032-0935 242:1<113 2015 242 425