caa a22 4500 606207538 CHVBK 20210128101017.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00425-015-2311-7 doi (NATIONALLICENCE)springer-10.1007/s00425-015-2311-7 MicroRNAs expression patterns in the response of poplar woody root to bending stress [Elektronische Daten] [Miriam Rossi, Dalila Trupiano, Manuela Tamburro, Giancarlo Ripabelli, Antonio Montagnoli, Donato Chiatante, Gabriella Scippa] Main conclusion : The paper reports for the first time, in poplar woody root, the expression of five mechanically-responsive miRNAs. The observed highly complex expression pattern of these miRNAs in the bent root suggest that their expression is not only regulated by tension and compression forces highlighting their role in several important processes, i.e., lateral root formation, lignin deposition, and response to bending stress. Mechanical stress is one of the major abiotic stresses significantly affecting plant stability, growth, survival, and reproduction. Plants have developed complex machineries to detect mechanical perturbations and to improve their anchorage. MicroRNAs (miRNAs), small non-coding RNAs (18-24 nucleotides long), have been shown to regulate various stress-responsive genes, proteins and transcription factors, and play a crucial role in counteracting adverse conditions. Several mechanical stress-responsive miRNAs have been identified in the stem of Populus trichocarpa plants subjected to bending stress. However, despite the pivotal role of woody roots in plant anchorage, molecular mechanisms regulating poplar woody root responses to mechanical stress have still been little investigated. In the present paper, we investigate the spatial and temporal expression pattern of five mechanically-responsive miRNAs in three regions of bent poplar woody taproot and unstressed controls by quantitative RT-PCR analysis. Alignment of the cloned and sequenced amplified fragments confirmed that their nucleotide sequences are homologous to the mechanically-responsive miRNAs identified in bent poplar stem. Computational analysis identified putative target genes for each miRNA in the poplar genome. Additional miRNA target sites were found in several mechanical stress-related factors previously identified in poplar root and a subset of these was further analyzed for expression at the mRNA or protein level. Integrating the results of miRNAs expression patterns and target gene functions with our previous morphological and proteomic data, we concluded that the five miRNAs play crucial regulatory roles in reaction woody formation and lateral root development in mechanically-stressed poplar taproot. Springer-Verlag Berlin Heidelberg, 2015 Mechanical stress nationallicence MiRNAs nationallicence Populus nigra nationallicence Reaction wood nationallicence Taproot nationallicence ABA : Abscisic acid nationallicence ABS : Above-bending sector nationallicence AP2 : APETALA 2 nationallicence BBP : Blue basic copper protein nationallicence BBS : Below-bending sector nationallicence BS : Bending sector nationallicence BTF3 : Basic transcription factor 3 nationallicence ERF : Ethylene responsive factor nationallicence GA : Gibberellic acid nationallicence GAI : Gibberellins insensitive nationallicence IAA : Indole-3-acetic acid nationallicence NAC : Nascent polypeptide associated complex nationallicence RGL1 : Repressor of ga1-3(RGA)-like 1 nationallicence SCR : Scarecrow nationallicence STK : Serine/threonine protein kinase nationallicence Rossi Miriam Dipartimento di Bioscienze e Territorio, University of Molise, C.da Fonte Lappone, 86090, Pesche (IS), Italy aut Trupiano Dalila Dipartimento di Bioscienze e Territorio, University of Molise, C.da Fonte Lappone, 86090, Pesche (IS), Italy aut Tamburro Manuela Dipartimento di Medicina e Scienze della Salute, University of Molise, 86100, Campobasso, Italy aut Ripabelli Giancarlo Dipartimento di Medicina e Scienze della Salute, University of Molise, 86100, Campobasso, Italy aut Montagnoli Antonio Dipartimento di Biotecnologie e Scienze della Vita, University of Insubria, 21100, Varese, Italy aut Chiatante Donato Dipartimento di Biotecnologie e Scienze della Vita, University of Insubria, 21100, Varese, Italy aut Scippa Gabriella Dipartimento di Bioscienze e Territorio, University of Molise, C.da Fonte Lappone, 86090, Pesche (IS), Italy aut Planta Springer Berlin Heidelberg 242/1(2015-07-01), 339-351 0032-0935 242:1<339 2015 242 425 https://doi.org/10.1007/s00425-015-2311-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00425-015-2311-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rossi Miriam Dipartimento di Bioscienze e Territorio, University of Molise, C.da Fonte Lappone, 86090, Pesche (IS), Italy aut NATIONALLICENCE 700 1- Trupiano Dalila Dipartimento di Bioscienze e Territorio, University of Molise, C.da Fonte Lappone, 86090, Pesche (IS), Italy aut NATIONALLICENCE 700 1- Tamburro Manuela Dipartimento di Medicina e Scienze della Salute, University of Molise, 86100, Campobasso, Italy aut NATIONALLICENCE 700 1- Ripabelli Giancarlo Dipartimento di Medicina e Scienze della Salute, University of Molise, 86100, Campobasso, Italy aut NATIONALLICENCE 700 1- Montagnoli Antonio Dipartimento di Biotecnologie e Scienze della Vita, University of Insubria, 21100, Varese, Italy aut NATIONALLICENCE 700 1- Chiatante Donato Dipartimento di Biotecnologie e Scienze della Vita, University of Insubria, 21100, Varese, Italy aut NATIONALLICENCE 700 1- Scippa Gabriella Dipartimento di Bioscienze e Territorio, University of Molise, C.da Fonte Lappone, 86090, Pesche (IS), Italy aut NATIONALLICENCE 773 0- Planta Springer Berlin Heidelberg 242/1(2015-07-01), 339-351 0032-0935 242:1<339 2015 242 425